We narrowed to 7,281 results for: Tet-on

1. PGK+ episome

   Plasmid

   #170041

   Purpose

   PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/TO-myc-BirA*-NES

   Plasmid

   #112714

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   HIV Rev nuclear export signal (NES)
   

   Expression

   Mammalian

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-FH-eGFP

   Plasmid

   #157734

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-eGFP
   

   Tags

   Flag-His8

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV+ episome

   Plasmid

   #169742

   Purpose

   CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAluYNF1

   Plasmid

   #50933

   Purpose

   Expresses dimeric AluY RNA in mammalian cells.

   Depositor

   Katharina Strub

   Article

   Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

   Insert

   AluYNF1
   

   Expression

   Mammalian

   Promoter

   7SL RNA promoter (pol III)

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pPBO.ACT003

   Plasmid

   #182713

   Purpose

   aTc inducible dCasRx-IF3

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   3x(GGGS)-Escherichia Coli Initiation Factor 3

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pTet

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos2-CD9-10

   Plasmid

   #57356

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   CD9 (CD9 Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pFAB808

   Plasmid

   #47853

   Purpose

   BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rrnB T1 terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLTetO8

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-TO-eGFP-HF

   Plasmid

   #157735

   Purpose

   mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   eGFP-HF
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV+ plasmid

    Plasmid

    #169740

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJB332

    Plasmid

    #115949

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 TetR-Gly9-DDX6 (contains SapI)
    

    Use

    Synthetic Biology

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTW122

    Plasmid

    #115962

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 5'-2xTetApt-mVenus-PEST
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSTAP-seq_human-4xUAS

    Plasmid

    #125150

    Purpose

    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Stap-seq screening vector

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2H12Matry-blind

    Plasmid

    #221181

    Purpose

    cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen-Matry(blind)
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE163

    Plasmid

    #118511

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Expression

    Bacterial

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJB556

    Plasmid

    #115966

    Purpose

    SGP insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 -98/15 SGP 2xTetApt-STOP
    

    Use

    Synthetic Biology

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCHOP10.pCDNA1

    Plasmid

    #21899

    Depositor

    David Ron

    Article

    Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

    Insert

    CHOP10 (Ddit3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pFAB815

    Plasmid

    #47854

    Purpose

    BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rnpB T1 terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO9

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183605

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE160

    Plasmid

    #118508

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Expression

    Bacterial

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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