We narrowed to 14,669 results for: Egf
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Plasmid#225436PurposeBeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.DepositorInsertBeYDV geminino 1.0
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4636
Plasmid#215264PurposeModule for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19DepositorInsertEtr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4541
Plasmid#215260PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the Red light-regulated Etr4:mDFR promoter and TNOS in and the constitutve expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertEtr4:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFPEasy9R
Plasmid#218291PurposePlasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.DepositorInsertpURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4QE2Crimson
Plasmid#218280PurposeIntroduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoterDepositorInsertpURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC2 CTF-FLAG
Plasmid#217685PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR2 (CELSR2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2356Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1318
Plasmid#217960PurposeExpression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
TagseGFPExpressionMammalianMutationL63A, F66A, I71APromoterCMVAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1317
Plasmid#217959PurposeExpression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
TagseGFPExpressionMammalianMutationGGG insertion between V65 and F66PromoterCMVAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1315
Plasmid#217957PurposeExpression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
TagseGFPExpressionMammalianMutationF84A, L92A, Y99APromoterCMVAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1314
Plasmid#217956PurposeExpression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
TagseGFPExpressionMammalianMutationGGG insertion between A95 and C96PromoterCMVAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lnig-INTR-GFP
Plasmid#208904Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFPDepositorInsertINTR
TagsEGFPExpressionMammalianAvailable SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-4 (b)
Plasmid#204435PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (c)
Plasmid#204430PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (d)
Plasmid#204431PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (e)
Plasmid#204432PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-5
Plasmid#204436PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-6
Plasmid#204437PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-7
Plasmid#204438PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
TagsEGFPExpressionMammalianMutationK819A:N820APromoterCMVAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-8
Plasmid#204439PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Control-2
Plasmid#204443PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Control-3
Plasmid#204444PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Control-4
Plasmid#204445PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-1
Plasmid#184567PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsmEGFPMutationreplaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…PromoterSFFVAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
TagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-loxP-canisNrg1
Plasmid#173849PurposeEncoding dog Nrg1 before Cre-mediated recombination.DepositorInsertA cDNA of canis Nrg1
ExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgfr
Plasmid#170303PurposeA knock-out vector for the mouse PtgfrDepositorInsertA gRNA targeting the mouse Ptgfr gene.
UseCRISPR and LentiviralAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only