We narrowed to 11,692 results for: NSI

1. LacI/109-pHG165c

   Plasmid

   #90058

   Purpose

   WT LacI polymorphism with T at position 109

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LacI/109
   

   Expression

   Bacterial

   Mutation

   109

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXW117As(RS-E11)

   Plasmid

   #123145

   Purpose

   Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J117, ParsR, PhrpLE, Pecf11

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVL94 - pOp4:VND7

   Plasmid

   #116016

   Purpose

   destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

   Depositor

   Thomas Greb

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
   

   Expression

   Plant

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_N_RAB11A

   Plasmid

   #111691

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   RAB11A (RAB11A Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA-G-intron(Neo)-T(FLLFLNL)

   Plasmid

   #40020

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdT-3Myc
   beta-globin intron
   Neo
   

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available Since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416 Gal TDP43 G294A

   Plasmid

   #27459

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Expression

   Yeast

   Mutation

   Glycine 294 mutated to Alanine (G294A)

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pOPTXcGMPRELUC

   Plasmid

   #68503

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
   

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

   Promoter

   OPTX promoter with cGMPRE

   Available Since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

8. phumanCas9 (GB0575)

   Plasmid

   #68206

   Purpose

   Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_PSTPIP1-1/1

   Plasmid

   #91453

   Purpose

   Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PSTPIP1-1/1 (PSTPIP1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sumo3 PolQM1 deltains3

    Plasmid

    #78459

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2497-2529

    Promoter

    T7

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMXs-1003

    Plasmid

    #31712

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-367 (Mir367 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pHH0103_PIK3R1-1/1

    Plasmid

    #91274

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R1-1/1 (PIK3R1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4-Tet-on-vsv-Incenp-EGFP

    Plasmid

    #108483

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTNos:BASTA:PNos (GB0023)

    Plasmid

    #68211

    Purpose

    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    bar gene
    

    Use

    Unspecified

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    PNos

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT250_(pCA-G-intron(Neo)-tTA2)

    Plasmid

    #36876

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR sgHAL

    Plasmid

    #102315

    Purpose

    genetic depletion of HAL

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgHAL (HAL Human)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-sh-Myocardin

    Plasmid

    #100769

    Purpose

    Lentiviral expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    Lenti-sh-Myocardin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_NEDD9-1/1

    Plasmid

    #91498

    Purpose

    Protein expression and purification of human SH3 domain construct NEDD9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NEDD9-1/1 (NEDD9 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGAL4-DBD-RUNX2+10A-IDR

    Plasmid

    #145251

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and RUNX2A+10A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2A+10A-IDR
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FN(9-10)-I27-CC

    Plasmid

    #85394

    Purpose

    MTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    Fibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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