We narrowed to 7,900 results for: sms

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pICE-EGFP-FLAG-Ku70siR-Mut6E

Plasmid

#46962

Purpose

For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Mut6E corresponding to K282E K287E T300E K331E K3…

Promoter

CMV Tet

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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NET37 pEGFP-N2 (607)

Plasmid

#61990

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET37 (KIAA1161 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
mKate2-P2A-APEX2-2xML1N(7Q)

Plasmid

#67666

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1Nmut*2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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miniSOG-Clathrin-15

Plasmid

#57764

Purpose

Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Clathrin (CLTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

Plasmid

#186745

Purpose

dCas9-10XGcn4-P2A-mCherry expression construct

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

Cas9-10XGcn4-P2A-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-H2B-C-10

Plasmid

#55090

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pEF1a_ TET1 CXXC only

Plasmid

#124084

Purpose

Expression of CXXC domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - CXXC domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

Plasmid

#185328

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_81-236-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 C548A

Plasmid

#126592

Purpose

Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C548A

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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miniSOG-Vimentin-7

Plasmid

#57779

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vimentin (VIM Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FLAG-SENP2 WT

Plasmid

#126591

Purpose

Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant to GAGGAGAUAUUCAGACAUU

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Tubulin-6

Plasmid

#55097

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
2.3 hA33 luciferase

Plasmid

#68148

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -2270 relative to ATG

Promoter

human GPA33 promoter

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
tdminiSOG-PCNA-19

Plasmid

#57806

Purpose

Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

PCNA (PCNA Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pN1_HsKIF1C(1-376)-NC-LZ-mNG

Plasmid

#253325

Purpose

mammalian expression of the motor domain (aa 1-376) of human KIF1C tagged with mNG. The NC and LZ sequences facilitate stable dimerization of the motor domains.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(1-376) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(349-1103)-mNG

Plasmid

#253327

Purpose

mammalian expression of the stalk+tail domains of human KIF1C (aa 349-1103) tagged with mNG.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(349-1103) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(886-1103)-mNG

Plasmid

#253328

Purpose

mammalian expression of the IDR domain of human KIF1C (aa 886-1103) tagged with mNG.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(886-1103) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pTRE3G-marmoset cGASdeltaN-HA

Plasmid

#186863

Purpose

Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Marmoset cGAS truncation mutant (160-547 a.a.) with C-terminal HA tag

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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