We narrowed to 11,684 results for: cel.2

1. pCSIIhyg-rtTA

   Plasmid

   #139480

   Purpose

   Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   rtTA, Tet-ON reverse transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a promoter

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PE2-VRQR

   Plasmid

   #159983

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-VRQR
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mOtop2_pcDNA3

   Plasmid

   #114678

   Purpose

   This construct was used to express mouse Otop2 in HEK-293 cells

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 2 (Otop2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCiPS-NIFV

   Plasmid

   #110807

   Purpose

   Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

   Depositor

   David Russell

   Article

   Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

   Insert

   Pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2 mutations in the DDE catalyic core motif of the…

   Promoter

   CMV

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-PT2A-GFP-iRFP670-Tdtomato

   Plasmid

   #111813

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   iRFP670
   Tdtomato
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-PT2A-iRFP670-GFP-Tdtomato

   Plasmid

   #111816

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   GFP
   Tdtomato
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HA-mIFITM1

   Plasmid

   #58415

   Purpose

   Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

   Insert

   IFITM1 (Ifitm1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Rap-MESA-20-M-Gal4

   Plasmid

   #84554

   Purpose

   MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   Rap-MESA-FRB-20-M-Gal4
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTubulin-BC2T

   Plasmid

   #108970

   Purpose

   expresses tubulin-BC2-tag in mammalian cells

   Depositor

   Ulrich Rothbauer

   Article

   Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

   Insert

   a-tubulin
   

   Use

   Tags

   BC2-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mNox1

    Plasmid

    #58340

    Purpose

    expresses mouse Nox1 in mammalian cells

    Depositor

    Botond Banfi, Karl-Heinz Krause

    Article

    Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

    Insert

    Mus musculus NADPH oxidase 1 alpha (Nox1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC_N_ETV7

    Plasmid

    #167756

    Purpose

    Expresses ETV7 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETV7 (ETV7 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVIM-BC2T

    Plasmid

    #108967

    Purpose

    expresses vimentin-BC2-tag in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    Vimentin
    

    Use

    Tags

    BC2T

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pfTRAP

    Plasmid

    #50048

    Purpose

    Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    plamodium falciparum TRAP (TRAP Synthetic)
    

    Use

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pfTRAP(VWA)

    Plasmid

    #50049

    Purpose

    Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    pfTRAP(vwa) (TRAP Synthetic)
    

    Use

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pEF-HA/Net1A

    Plasmid

    #28262

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1A (Net1 Mouse)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pP2AeGFP-Mars1Cy

    Plasmid

    #75223

    Purpose

    Mammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFP

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    EGFP-p2a-Mars1Cy
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-MLG-HMF-LST2

    Plasmid

    #220786

    Purpose

    Expresses N-terminal His10-Myc-FLAG-LST2 in mammalian cells

    Depositor

    Timm Maier

    Article

    Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    LST2 (ZFYVE28 Human)
    

    Use

    Tags

    10xHis-Myc-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAceBAC2-MLG-HMF-LST2

    Plasmid

    #220787

    Purpose

    Expresses N-terminal His10-Myc-FLAG-LST2 in insect cells

    Depositor

    Timm Maier

    Article

    Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    LST2 (ZFYVE28 Human)
    

    Use

    Tags

    10xHis-Myc-FLAG

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-KG/Net1A

    Plasmid

    #28261

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1A (Net1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pLenti Lifeact-EGFP BlastR

    Plasmid

    #84383

    Purpose

    Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti Lifeact-iRFP670 BlastR

    Plasmid

    #84385

    Purpose

    Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV7.1_3xFLAG-TAOK1

    Plasmid

    #172991

    Purpose

    constitutive expression of FLAG-tagged TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK1 (TAOK1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PCBP1_Full-length_pGEX

    Plasmid

    #135108

    Purpose

    Recombinant expression of PCBP1 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    PCBP1 (PCBP1 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-mGold-Mito-N-7

    Plasmid

    #158007

    Purpose

    Visualization of mitochondria in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOPINK-OTUB2 (full-length, aa 1-234)

    Plasmid

    #61421

    Purpose

    Expresses human OTUB2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pINDUCER21-puro_TAOK1

    Plasmid

    #209777

    Purpose

    doxycycline-inducible expression of TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

    Plasmid

    #105209

    Purpose

    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RBM6_RRM_pGEX

    Plasmid

    #135157

    Purpose

    Recombinant expression of RBM6 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBM6 (RBM6 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

    Plasmid

    #61938

    Purpose

    Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF2) (UCHL5 Human)
    hNFRKB (39-156) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 2-38 dele…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid

    #170808

    Purpose

    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Inserts

    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Synthetic, Mustard Weed, S. aureus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous mutation in PYL1 to remove XhoI site (…

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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