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pET-11a (GB1 WT - A34F/V39L)

Plasmid

#136610

Purpose

Expresses GB1 WT double mutant (A34F/V39L)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

GB1 WT double mutant (A34F/V39L)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: A34F - V39L

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_007

Plasmid

#123788

Purpose

Encodes bacterial light-activated adenylyl cyclase (bPAC) as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

bPAC

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_011

Plasmid

#123726

Purpose

Encodes HA-tagged PIF6 fused to Tet Repressor protein as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TETR::PIF6::HA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0747

Plasmid

#133481

Purpose

Expression in fission yeast from promoter ppom1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0748

Plasmid

#133482

Purpose

Expression in fission yeast from promoter ppak1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0605

Plasmid

#133503

Purpose

Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0746

Plasmid

#133480

Purpose

Expression in fission yeast from promoter prga3 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB6637

Plasmid

#106164

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC104650.1-1/1

Plasmid

#91541

Purpose

Protein expression and purification of human SH3 domain construct AC104650.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC104650.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC093799.1-1/1

Plasmid

#91495

Purpose

Protein expression and purification of human SH3 domain construct AC093799.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC093799.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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