We narrowed to 16,181 results for: nes
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Plasmid#145784PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).DepositorInsertCapsid
UseLentiviral and RetroviralExpressionMammalianMutationChanged Capsid residues Glutamines 63 and 67 to a…Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-DIO-tdTomato-f
Plasmid#127092PurposeAn AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomatoDepositorInserttdTomato-f
UseAAVTagsfarnesylation signal from c-Ha-RasExpressionMammalianAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9-p (with stop codon)
UseCRISPRAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PLEKHS1
Plasmid#110509PurposeFluorescent PLEKHS1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLEKHS1 (C10orf81 Human)
TagsEGFPExpressionMammalianAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)
Plasmid#50032PurposeGalactose-inducible expression of ER-luminal S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
TagsHA-HDELExpressionYeastPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRM055 His-TEV-Cas9 6xNLS 0C
Plasmid#196246PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
ExpressionBacterialPromoterT7Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
K406
Plasmid#129764PurposeExpresses Drosophila Kinesin-1 truncated after neck-coil at 406DepositorAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPC-JAG1-mVenus
Plasmid#171170PurposeRetroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).DepositorAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215328PurposeLentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertFOXJ1 Promoter
UseLentiviral and LuciferaseExpressionMammalianPromoterFOXJ1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
IDG_KCNAB2_OE_1
Plasmid#161647PurposeOverexpresses KCNAB2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKCNAB2 (KCNAB2 Human)
TagsV5ExpressionMammalianAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ABCE1-Halo
Plasmid#175335PurposeProduction of HaloTag fusion proteinDepositorPublicationAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
Plasmid#159803PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ABE8.8-d
Plasmid#136295Purposeexpresses ABE8.8-d in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE8.8-d
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-K2-2xCSTREP
Plasmid#136149PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertK2
ExpressionMammalianPromoterCMVAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
mOPN-PB0
Plasmid#106449PurposeBacterial expression of murine osteopontinDepositorAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRAPPC8_pLX307
Plasmid#98375PurposeLentiviral expression of TRAPPC8DepositorAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(D323A/D325A)
Plasmid#86630PurposeExpresses eGFP-tagged human Akt1(D323A/D325A) mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt1(D323A/D325A) (AKT1 Human)
ExpressionMammalianAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only