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CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini DM, Cantor JR
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
163444pLJC5-Rap2A-3xFLAGlentiviral overexpression vector for human Rap2A
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163445pLJC6-Rap2A-3xFLAGlentiviral overexpression vector for human Rap2A
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163446pLJC2-GPT1-3xFLAGlentiviral overexpression vector for human GPT1
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163447pLJC2-GPT2-3xFLAGlentiviral overexpression vector for human GPT2
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163448pLJC6-GPT1-3xFLAGlentiviral overexpression vector for human GPT1
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163449pLJC6-GPT2-3xFLAGlentiviral overexpression vector for human GPT2
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163450pLJC6-GLS-3xFLAGlentiviral overexpression vector for human GLS
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163451pLJC6-HK2-3xFLAGlentiviral overexpression vector for human HK2
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163452pLJC6-NADK-3xFLAGlentiviral overexpression vector for human NADK
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163453pLJC6-METAP1-3xFLAGlentiviral overexpression vector for human METAP1
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163454pLJC6-EVempty vector control for pLJC6
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163455pLentiCRISPR-v1-sgGPT2_5lentiviral vector expressing Cas9 and an sgRNA targeting GPT2
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163456pLentiCRISPR-v1-sgGPT2_9lentiviral vector expressing Cas9 and an sgRNA targeting GPT2
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163457pLentiCRISPR-v1-sgMPC2_7lentiviral vector expressing Cas9 and an sgRNA targeting MPC2
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163458pLentiCRISPR-v1-sgMPC2_9lentiviral vector expressing Cas9 and an sgRNA targeting MPC2
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163459pLentiCRISPR-v1-sgGLS_2lentiviral vector expressing Cas9 and an sgRNA targeting GLS
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163460pLentiCRISPR-v1-sgGLS_6lentiviral vector expressing Cas9 and an sgRNA targeting GLS
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163461pLentiCRISPR-v1-sgHK2_2lentiviral vector expressing Cas9 and an sgRNA targeting HK2
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163462pLentiCRISPR-v1-sgNADK_1lentiviral vector expressing Cas9 and an sgRNA targeting NADK
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163463pLentiCRISPR-v1-sgMETAP1_2lentiviral vector expressing Cas9 and an sgRNA targeting METAP1
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Recombinant Antibodies from Article