CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini DM, Cantor JR
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005. PubMed Article
Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini DM, Cantor JR
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163444 | pLJC5-Rap2A-3xFLAG | lentiviral overexpression vector for human Rap2A |
| 163445 | pLJC6-Rap2A-3xFLAG | lentiviral overexpression vector for human Rap2A |
| 163446 | pLJC2-GPT1-3xFLAG | lentiviral overexpression vector for human GPT1 |
| 163447 | pLJC2-GPT2-3xFLAG | lentiviral overexpression vector for human GPT2 |
| 163448 | pLJC6-GPT1-3xFLAG | lentiviral overexpression vector for human GPT1 |
| 163449 | pLJC6-GPT2-3xFLAG | lentiviral overexpression vector for human GPT2 |
| 163450 | pLJC6-GLS-3xFLAG | lentiviral overexpression vector for human GLS |
| 163451 | pLJC6-HK2-3xFLAG | lentiviral overexpression vector for human HK2 |
| 163452 | pLJC6-NADK-3xFLAG | lentiviral overexpression vector for human NADK |
| 163453 | pLJC6-METAP1-3xFLAG | lentiviral overexpression vector for human METAP1 |
| 163454 | pLJC6-EV | empty vector control for pLJC6 |
| 163455 | pLentiCRISPR-v1-sgGPT2_5 | lentiviral vector expressing Cas9 and an sgRNA targeting GPT2 |
| 163456 | pLentiCRISPR-v1-sgGPT2_9 | lentiviral vector expressing Cas9 and an sgRNA targeting GPT2 |
| 163457 | pLentiCRISPR-v1-sgMPC2_7 | lentiviral vector expressing Cas9 and an sgRNA targeting MPC2 |
| 163458 | pLentiCRISPR-v1-sgMPC2_9 | lentiviral vector expressing Cas9 and an sgRNA targeting MPC2 |
| 163459 | pLentiCRISPR-v1-sgGLS_2 | lentiviral vector expressing Cas9 and an sgRNA targeting GLS |
| 163460 | pLentiCRISPR-v1-sgGLS_6 | lentiviral vector expressing Cas9 and an sgRNA targeting GLS |
| 163461 | pLentiCRISPR-v1-sgHK2_2 | lentiviral vector expressing Cas9 and an sgRNA targeting HK2 |
| 163462 | pLentiCRISPR-v1-sgNADK_1 | lentiviral vector expressing Cas9 and an sgRNA targeting NADK |
| 163463 | pLentiCRISPR-v1-sgMETAP1_2 | lentiviral vector expressing Cas9 and an sgRNA targeting METAP1 |