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pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

Plasmid

#200541

Purpose

mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA-FRT-acry:venus-pA-FRT

Plasmid

#200543

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry:venus-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_CreER-pA-FRT-acry:mCherry-pA-FRT

Plasmid

#200544

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry: mCherry -pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_P2A-CreER-pA ORF1 FRT-acry:mCherry-pA-FRT

Plasmid

#200545

Purpose

mini circle middle entry plasmid for P2A-CreER-pA (ORF 1) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_P2A-CreER-pA ORF2 FRT-acry:mCherry-pA-FRT

Plasmid

#200546

Purpose

mini circle middle entry plasmid for P2A-CreER-pA (ORF 2) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_P2A-CreER-pA ORF3 FRT-acry:mCherry-pA-FRT

Plasmid

#200547

Purpose

mini circle middle entry plasmid for P2A-CreER-pA (ORF 3) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1

Plasmid

#200529

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2

Plasmid

#200530

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3

Plasmid

#200531

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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MCUb-mVenus-pLJM

Plasmid

#236789

Purpose

MCUB tagged with mVenus for FRET / MW change for stoichiometry

Depositor

Dipayan Chaudhuri

Article

Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

Insert

MCUB (MCUB Human)

Use

Lentiviral

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFHΔ36-72

Plasmid

#232060

Purpose

Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Heavy tail domain lacking residues 36-72

Tags

6xHis

Expression

Bacterial

Mutation

added N-terminal tryptophan and tyrosine, and cys…

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFHΔ462-638

Plasmid

#232061

Purpose

Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Heavy tail domain lacking residues 462-638

Tags

6xHis

Expression

Bacterial

Mutation

added N-terminal tryptophan and tyrosine, and cys…

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFMtail

Plasmid

#232056

Purpose

Expresses construct for surface tethering: R. norvegicus NFM tail domain

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Medium tail domain

Tags

6xHis, thrombin

Expression

Bacterial

Mutation

cDNA codon-optimized for E. coli; added N-termina…

Promoter

T7

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFHtail

Plasmid

#232057

Purpose

Expresses construct for surface tethering: R. norvegicus NFH tail domain

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Heavy tail domain

Tags

6xHis

Expression

Bacterial

Mutation

added N-terminal tryptophan and tyrosine, and cys…

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

Plasmid

#232128

Purpose

A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA2 (GATA2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#232124

Purpose

A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#232123

Purpose

A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#232129

Purpose

A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

Plasmid

#232131

Purpose

A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF2 (FOXF2 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

Plasmid

#232130

Purpose

A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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