We narrowed to 4,048 results for: GAST

1. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199196

   Purpose

   Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUAST-attB-3xHA-V5-RpL10Ab

   Plasmid

   #125223

   Purpose

   3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Use

   Tags

   3x HA, V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dox-Akt

   Plasmid

   #207328

   Purpose

   PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

   Depositor

   Jared Toettcher

   Article

   Underhill et al Development. 2023 Aug 15;150(16):dev2016

   Insert

   rac protein kinase-alpha (AKT1 Human)
   

   Use

   Tags

   myristoylation tag - BFP

   Expression

   Bacterial

   Mutation

   range: 129 - 480

   Promoter

   Tet Response Element

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS5-3HA/p14ARF

   Plasmid

   #78764

   Purpose

   To overexpress p14ARF in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

   Insert

   p14ARF (CDKN2A Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

   Plasmid

   #201685

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

   Plasmid

   #201686

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. KHBD00476

   Plasmid

   #39585

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG5838 (Dref Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. UAS-dsarm(E)

   Plasmid

   #187870

   Purpose

   Gal4/UAS expression of dSarm(isoE)

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   dSarm isoform E (Sarm Fly)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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