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pK1_PHLT_R1_E2Crimson

Plasmid

#250886

Purpose

E2-Crimson expressed under PHLT synthetic promoter; component of BNeuAS4; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PHLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHL_R1_EGFP

Plasmid

#250888

Purpose

EGFP expressed under PHL synthetic promoter ; part of BNeu AS8; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHL

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_E2Crimson

Plasmid

#250882

Purpose

E2-Crimson expression under PLT synthetic promoter; part of BNeu AS1*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PR_R1_E2Crimson

Plasmid

#250896

Purpose

E2-Crimson expressed under PR promoter; component of Bneu AS10; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PR

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pK1_PLT_R1_EGFP

Plasmid

#250881

Purpose

EGFP expression under PLT synthetic promoter; component of BNeuAS1; p15A origin

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pK1_PHT_R4_EGFP

Plasmid

#250889

Purpose

EGFP expressed under PHT synthetic promoter and regulated by RBS 'R4'; part of BNeuAS9; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
HRPEadh-pAG303

Plasmid

#249735

Purpose

Derived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG305

Plasmid

#249734

Purpose

Derived from pAG305GPD-ccdb (#14138); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSBinit-Syb-BC10(Y35A/Y56A/R60A/I61T/H63A/T105A/Y107A/W108S)

Plasmid

#250415

Purpose

Expression of Anti-SLC66A2 Sybody Non-Binding Variant in Bacteria

Depositor

Simon Newstead

Insert

Syb-BC10

Use

Affinity Reagent/ Antibody

Tags

Myc-6xHis and PelB

Expression

Bacterial

Mutation

CDR1(Y35A)/CDR2(Y56A/R60A/I61T/H63A)/CDR3(T105A/Y…

Promoter

araBAD

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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BeYDV-RT-pUPD2

Plasmid

#240747

Purpose

BeYDV viral vector containing a repair template.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BeYDV:RT

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTV-CS-pUPD2

Plasmid

#240746

Purpose

BCTV complementary sense ORFs with C1 Goldenbraid grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV CS

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔIR-pUPD2

Plasmid

#240717

Purpose

BCTV complementary sense ORFs lacking the IR. Has C1 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV CS

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVS-pUPD2

Plasmid

#240718

Purpose

BCTV IR lacking virion sense ORFs.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV IR

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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RT B2-B6-pUPD2

Plasmid

#240719

Purpose

GUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B2-B6 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
RT B6-pUPD2

Plasmid

#240720

Purpose

GUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B6 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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ZFN-pUPD2

Plasmid

#240721

Purpose

ZFN that targets the broken GUS:NPTII reporter with B2-B5 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

ZFN

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAG304-ccdbNLUC

Plasmid

#249736

Purpose

Derived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiae

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pACYC-PJ23100-dpGFP

Plasmid

#249257

Purpose

An avGFP mutant designed by DeepDE that achieved a remarkable 74.3-fold increase in activity over four rounds of evolution, far surpassing the benchmark superfolder GFP.

Depositor

Xiaofeng Yang

Article

Li et al iScience. 2025 Aug 7;28(9):113324. doi:

Insert

dpGFP

Expression

Bacterial

Promoter

PJ23100

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1370_pTet-ECOR31_Hachiman

Plasmid

#223364

Purpose

Expression of ECOR31 Hachiman under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1464_pTet-ECOR31Ham_DELhamA_Mut

Plasmid

#223365

Purpose

Expression of ECOR31 Hachiman HamB (∆HamA) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamB (∆HamA)

Expression

Bacterial

Mutation

∆HamA

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge