We narrowed to 11,760 results for: pcdna

1. pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

   Plasmid

   #69907

   Purpose

   Expresses a circular RNA containing the 5' half of GFP in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   GFP, ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

   Plasmid

   #111459

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523), C643A

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. (363) pcDNA3.1-Nup62-Ubc-Flag-EPEA

   Plasmid

   #168098

   Purpose

   Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   Nup62-Ubc-Flag-EPEA
   

   Use

   Tags

   EPEA-tag, Flag-tag, and Ubc-Tag

   Expression

   Mammalian

   Mutation

   P229A, S283T on Nup62 native sequence

   Promoter

   T7/cmv

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

   Plasmid

   #169992

   Purpose

   Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   TEV-siriusGFP-FLAG

   Expression

   Mammalian

   Mutation

   S297A

   Promoter

   CMV

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CFP(159-238)-beta-1 in pcDNAI/Amp

   Plasmid

   #55592

   Purpose

   A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
   

   Use

   Tags

   CFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   CFP(159-238) includes a substitution of His for A…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA 3.1(-) rat C/EBP beta (LIP)

   Plasmid

   #12562

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta LIP (Cebpb Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-eGFP-NLS-RNF168 delta RING

   Plasmid

   #133983

   Purpose

   mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of RING; siRNA resistant sequence: GAGGA…

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pcDNA4-TO-myc-His Trask [YdeltaF] mutant

   Plasmid

   #31769

   Depositor

   Mark Moasser

   Article

   Spassov et al PLoS One. 2011 Apr 29;6(4):e19154.

   Insert

   Trask (CDCP1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Y707/734/743F

   Promoter

   CMV

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5FRT/Flag-hINO80 HSA (212-526)

   Plasmid

   #29443

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Chen et al J Biol Chem. 2011 Feb 8. ():.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 212-526 (a.a.)

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

10. YFP(159-238)Stop in pcDNA1/amp

    Plasmid

    #60891

    Purpose

    This vector attaches YFP(159-238) to the C-terminus of a protein.

    Depositor

    Catherine Berlot

    Insert

    YFP(159-238)Stop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcdna3 Flag Jip3b(T266A, T276A, T287A)

    Plasmid

    #58924

    Purpose

    interacts with MAPK signaling module

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

    Insert

    mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed T266A, T276A, T287A

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127316

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.2 mir-1-1 reporter hsa-mir-138-2

    Plasmid

    #46677

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-138-2 (MIR138-2 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. XE254 pCDNA3.1(zeo)-hDsh3-497-716

    Plasmid

    #16755

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-497-716-Dsh3 (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa 497-716

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127318

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt Mouse, HIV)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3 Flag MKK3b(Glu, delete LXL)

    Plasmid

    #50450

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted L25-I27, changed Serine 218 to Glutamic A…

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

    Plasmid

    #69894

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 FLAG mTOR 1968-2549 S2035T

    Plasmid

    #23000

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 7. ()

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T aa 1968-2549 only

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only