We narrowed to 12,157 results for: pcdna

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pcDNA3.2 mir-1-1 reporter cel-mir-50

Plasmid

#46656

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-50

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-40

Plasmid

#46653

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-40

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-p300 Core (C1204R)

Plasmid

#61361

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 55

Plasmid

#59824

Purpose

Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 55

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b(C631S, C643S and C699T)

Plasmid

#52128

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

C631S, C643S and C699T

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)

Plasmid

#52007

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF2,3mut

Expression

Mammalian

Mutation

mutated start codons for ORF3 and 4

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A,M142A(KaganF62)

Plasmid

#52002

Purpose

Expresses the human MAVS CDS containing two point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-5'UTR-MAVS-ORF1(KaganE49)

Plasmid

#52006

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF1mut

Expression

Mammalian

Mutation

mutation removing the start codon for ORF1

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100/125L-siResist

Plasmid

#49855

Purpose

Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100 and Methionine 125 mutated to Leuci…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal F423R/V435R/W427R

Plasmid

#47351

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

F423R/V435R/W427R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-Med26-NTD- K74A K75A

Plasmid

#49252

Purpose

Mammalian expression of flag-tagged human Med26-NTD- K74A K75A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

K74A, K75A, aa 1-339

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter cel-mir-34

Plasmid

#46652

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-34

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-60

Plasmid

#46658

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-60

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-mir-59

Plasmid

#46657

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-59

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-mir-46

Plasmid

#46655

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-46

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter dme-let-7

Plasmid

#46662

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-let-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter dme-mir-34

Plasmid

#46665

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-34

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-mir-1

Plasmid

#46651

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-lsy-6

Plasmid

#46650

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lsy-6

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter cel-lin-4

Plasmid

#46649

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-lin-4

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge