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LEA1_APHAV_Chakrabortee pSecTag (pBS0254)

Plasmid

#185143

Purpose

For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

LEA1_APHAV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf269

Plasmid

#12957

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf269

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-TET1_CD

Plasmid

#83570

Purpose

Expresses the catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-ZNF598

Plasmid

#141191

Purpose

Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAG876 pDisplay-pFAST

Plasmid

#172868

Purpose

expresses pFAST at the surface of mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST-PDGFR

Use

Tags

IgK leader sequence (N terminal on insert) and My…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGG-9.47_VP1,3 #2515

Plasmid

#74237

Purpose

Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-WT-VLCAD-FLAG

Plasmid

#55823

Purpose

Expresses WT-VLCAD in mammalian cells

Depositor

Yoshimi Homma

Article

Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

Insert

Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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Ana GvpC-SpyTag-His in pET28a

Plasmid

#85737

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (fused to SpyTag and His-tagged)

Use

Tags

His-tag and SpyTag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-Ubl-sr1(SIRPT1)

Plasmid

#75298

Purpose

Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 1-339

Use

Tags

GST-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET21b-LOV-ipaA

Plasmid

#40236

Depositor

Brian Kuhlman

Article

Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

Insert

LOV-ipaA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

Promoter

T7

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 Wt

Plasmid

#29552

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
WT N-His Ana GvpC in pET28a

Plasmid

#153294

Purpose

Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (N-terminal his-tagged)

Use

Tags

His-tag

Expression

Bacterial

Mutation

Added a hexahistidine tag after the methionine at…

Promoter

T7

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAR-9.47_VP1,3

Plasmid

#74240

Purpose

Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAG470 LifeAct-iFAST

Plasmid

#130821

Purpose

Expresses LifeAct-iFAST in mammalian cells (actin labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

LifeAct-iFAST

Use

Tags

myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAG498 MAP4-iFAST

Plasmid

#130820

Purpose

Expresses MAP4-iFAST in mammalian cells (microtubule labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

MAP4-iFAST

Use

Tags

Myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-TEV

Plasmid

#153296

Purpose

Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

TEV-sensitive Gas vesicle protein C (his-tagged)

Use

Tags

His-tag

Expression

Bacterial

Mutation

1) 21 bp TEV recognition and cleavage site flanke…

Promoter

T7

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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