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We narrowed to 17,734 results for: STO

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pET28-His6-TEV-Grb2-SH2-mCherry-AviTag

Plasmid

#214231

Purpose

Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Grb2 SH2 domain (GRB2 Human)

Tags

6xHis, AviTag, and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Lck-SH2-AviTag

Plasmid

#214203

Purpose

Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Lck kinase SH2 domain (LCK Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Blk-SH2-AviTag

Plasmid

#214204

Purpose

Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Blk kinase SH2 domain (BLK Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Hck-SH2-AviTag

Plasmid

#214206

Purpose

Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

p60-HCK (HCK Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-BRAF-V600E-IRES-mCherry

Plasmid

#221026

Purpose

Fluorescent reporter for expressing a segment of BRAF-V600E CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

BRAF V600E (BRAF Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235301

Purpose

ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

Plasmid

#222288

Purpose

Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

Depositor

Anne Straube

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Insert

KIF1C (KIF1C Human)

Tags

6xHis, GFP, and GST

Expression

Bacterial

Mutation

stalk region only amino acids 642 to 922

Promoter

T7

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

Plasmid

#222289

Purpose

Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

Depositor

Anne Straube

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Insert

KIF1C (KIF1C Human)

Tags

6His and GST

Expression

Bacterial

Mutation

stalk region only amino acids 642 to 922

Promoter

T7

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_R2L2

Plasmid

#188049

Purpose

Expresses His8-FLI1_DBD_R2L2(276-399_R337L_R340L_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated R337L…

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD_deltaAlpha4

Plasmid

#188050

Purpose

Expresses His8-FLI1_DBD_deltaAlpha4(276-361) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 362-452

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-ERG_DBD(306-429_F392A)

Plasmid

#188051

Purpose

Expresses His8-ERG_DBD(306-429_F392A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

ERG (ERG Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-305, 430-479, mutated F392A

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-FLI1_DBD(276-399_F362A)

Plasmid

#188047

Purpose

Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated F362A

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2-bGH

Plasmid

#235300

Purpose

ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235302

Purpose

ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-HP1a-gRNA-2

Plasmid

#237635

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HP1a gRNA (CBX5 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX1-KS

Plasmid

#237679

Purpose

For overexpression of mEGFP-SS18-SSX1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX1-KS (SS18, SSX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX1

Plasmid

#237678

Purpose

For overexpression of mEGFP-SS18-SSX1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX1 (SS18, SSX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX2-KS

Plasmid

#237680

Purpose

For overexpression of mEGFP-SS18-SSX2-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX2-KS (SS18, SSX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-HP1a-gRNA-1

Plasmid

#237634

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to HP1a locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HP1a gRNA (CBX5 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-TCOF1-gRNA

Plasmid

#237633

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to TCOF1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TCOF1 gRNA (TCOF1 Human)

Use

CRISPR

Tags

mCherry

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-FUS-repair-tempate

Plasmid

#237684

Purpose

For knock-in msfGFP to FUS locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

FUS (FUS Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2x3HA-T2A-Isl1

Plasmid

#233161

Purpose

Retroviral expression of mNgn2x3HA and Isl1

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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MKC84_HBB(HBA1reg-HBA1)

Plasmid

#232410

Purpose

AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC83_HBB(HBBreg-HBA1)

Plasmid

#232409

Purpose

AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-mEtfdh

Plasmid

#232314

Purpose

Expression of mouse mutant Etfdh

Depositor

Ivan Topisirovic

Article

Papadopoli et al bioRxiv 2024.10.25.620155

Insert

Etfdh (Etfdh Mouse)

Use

Lentiviral

Mutation

PAM Sequence Mutations: (G36C, T39C, C42T, C72T, …

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-Isl1-T2A-mLhx3

Plasmid

#233160

Purpose

Retroviral expression of Isl1 and Lhx3 for motor neuron specification

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-T2A-mLhx3 (Isl1, Lhx3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2x3HA-T2A-mLhx3

Plasmid

#233162

Purpose

Retroviral expression of mNgn2x3HA and Lhx3

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-T2A-Lhx3 (Neurog2, Isl1 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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HNRNPA1-miRFP670-TRF1

Plasmid

#227384

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric HNRNPA1 IDR (TERF1, HNRNPA1 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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macroH2A2-GFP (pc2191)

Plasmid

#223709

Purpose

Expression of macroH2A2 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A2-GFP (MACROH2A2, H2AFY2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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