We narrowed to 10,337 results for: plasmids 101

1. pyc111f

   Plasmid

   #158780

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6f-RPL10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. (SUMO)10-(SIM)5

   Plasmid

   #126866

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-Puro-TNNT2-Neo

   Plasmid

   #214013

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP1594_2x_NLS

   Plasmid

   #110625

   Purpose

   Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   None
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pyc111m

   Plasmid

   #158779

   Purpose

   ribosome-tagged, Cre-dependent GCaMP for soma localization

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   GCaMP6m-RPL10a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

   Plasmid

   #83491

   Purpose

   cre-dependent DN-AMPK under EF1a promoter

   Depositor

   Dong Kong

   Article

   Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

   Insert

   DN-AMPK-2A-mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   K45R

   Promoter

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. anti-Notch2_B9-pBIOCAM5

   Plasmid

   #39348

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Human, Mouse)
   

   Use

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-human NMyc-humanER-IRES-mCherry

   Plasmid

   #73571

   Purpose

   Mammalian retroviral expression of NMyc-ER

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   NMyc (MYCN Human)
   

   Use

   Retroviral

   Tags

   Human ER and IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAGH10 - RlucFluc minus 1 HIV-1

   Plasmid

   #198224

   Purpose

   Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

   Depositor

   Isha Jain

   Article

   Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

   Inserts

   Renilla luciferase
   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

    Plasmid

    #187358

    Purpose

    Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    HPC-4 antibody heavy chain, and 6xHIS tag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

    Plasmid

    #135412

    Purpose

    simultaneous optical recording and manipulation of electrical activity in neurons

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    SomArchon-EGFP-P2A-stGtACR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-hCMV1-hNANOG-IV

    Plasmid

    #60432

    Purpose

    tet-inducible human NANOG

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    NANOG (NANOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-Flex-mRuby3-2A

    Plasmid

    #203846

    Purpose

    Neuron specific expression of a Cre-dependent mRuby3

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry

    Plasmid

    #83490

    Purpose

    cre-dependent CA-AMPK under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    CA-AMPK-2A-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    H150R

    Promoter

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

    Plasmid

    #83489

    Purpose

    cre-dependent PAK1-AID under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    PAK1-AID-ires-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK, U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-HCF-1rep1E10A

    Plasmid

    #84607

    Purpose

    Plasmid contains HCF-1rep1E10A with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1E10A (HCFC1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-HCF-1rep1wt

    Plasmid

    #84606

    Purpose

    Plasmid contains HCF-1rep1wt with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1wt (HCFC1 Human)
    

    Use

    Tags

    GST-TEV and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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