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pST_DHN_TaDHN5 (pBS0801)

Plasmid

#185262

Purpose

For the mammalian expression of the durm wheat protein DHN_TaDHN5. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_TaDHN5

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_OsLEA5 (pBS0793)

Plasmid

#185259

Purpose

For the mammalian expression of the rice plant protein DHN_OsLEA5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_OsLEA5

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1957

Plasmid

#155151

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Publication

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

added Streptavidin tag on MlaF N-terminus

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AAEL012356 AstC R

Plasmid

#115401

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Publication

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL012356 AstC R (LOC5576162 Aedes aegypti)

Expression

Mammalian

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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MPS-1-cmyc

Plasmid

#113059

Purpose

Expresses worm Myc-tagged MPS-1 in mammalian cells

Depositor

Federico Sesti

Publication

Wang et al Biophys J. 2007 Nov 1;93(9):3083-91. doi

Insert

MiRP K+ channel accessory Subunit 1 (mps-1 Nematode)

Tags

c-Myc

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-1547

Plasmid

#82434

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO1 in mammalian cells

Depositor

Marina Chekulaeva

Publication

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO1

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh-1548

Plasmid

#82435

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

Depositor

Marina Chekulaeva

Publication

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO2

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pV1338

Plasmid

#111443

Purpose

Solo vector pV1326 + sgScADE2

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2

Use

CRISPR

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pGEM-T_vgll4a

Plasmid

#254513

Purpose

Partial vgll4a cDNA. For in vitro transcription of ISH probe

Depositor

Virginie Lecaudey

Publication

Lardennois et al bioRxiv 2025.05.08.652796

Insert

vgll4a (vgll4a Zebrafish)

Use

Zebrafish expression

Promoter

T7

Available since

Sept. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_vgll4l

Plasmid

#254507

Purpose

Untagged zebrafish vgll4l. For in vitro transcription (SP6) and RNA injection

Depositor

Virginie Lecaudey

Publication

Lardennois et al bioRxiv 2025.05.08.652796

Insert

vgll4l (vgll4l Zebrafish)

Use

Zebrafish expression

Promoter

SP6

Available since

June 25, 2026

Availability

Academic Institutions and Nonprofits only

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pEM120

Plasmid

#250588

Purpose

H2Apr-SAS6-mClover3-SAS6ter

Depositor

Lillian Fritz-Laylin

Publication

Medina et al Curr Biol. 2026 Jan 19;36(2):338-354.e5.

Insert

H2Apr-SAS6-mClover3-SAS6ter

Use

Agrobacterium binary plasmid

Tags

mClover3

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_NUP62-Fv_EGFP3

Plasmid

#252749

Purpose

Expression of NUP62 with three tandem EGFPs. FV is a modified FKBP domain (aka DmrB) whose dimerization is induced by addition of Rapalog2. Blocks nulcear pore upon addition of Rapalog2

Depositor

Kristen Verhey

Publication

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

FV, 3xEGFP

Expression

Mammalian

Available since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_NUP62-EGFP3

Plasmid

#252748

Purpose

Expression of NUP62 with three tandem EGFPs.

Depositor

Kristen Verhey

Publication

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

E3xGFP

Expression

Mammalian

Available since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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2xPeptide 8_Vps4p_L7C_A43C

Plasmid

#203358

Purpose

Vps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids

Depositor

Chris Hill

Publication

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

2xPeptide 8 and 6x-HIS-TEV

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCas-mut4_1_2_VA

Plasmid

#186441

Purpose

Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Publication

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12b(PI)

Plasmid

#186451

Purpose

Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Publication

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b (PI)

Use

CRISPR

Mutation

R122A, G143P

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAF9D94 (pAF10C6)

Plasmid

#185848

Purpose

Expressing artificial trans-repressor in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>CYC8>PABF1

Expression

Yeast

Mutation

None

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_93-127 (pBS0735)

Plasmid

#185231

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_93-127

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q7JLY3_CAEEL_48-124 (pBS0738)

Plasmid

#185232

Purpose

For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q7JLY3_CAEEL_48-124

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_51-127_noLink (pBS0733)

Plasmid

#185229

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-127_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-127_noLink

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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