We narrowed to 18,729 results for: Mut

Showing: 6601 - 6620 of 18729 results

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pcDNA-Flag-LmoCas5

Plasmid

#126484

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pVC297 VEGF Site#1

Plasmid

#47505

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 1 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (4SP/AP+AAVA)

Plasmid

#14955

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP+AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660E

Plasmid

#45705

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660E

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
HDM_omicron_B11529_IDTDNA

Plasmid

#204142

Purpose

Expression plasmid for SARS-CoV-2 BA.1 variant spike

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#196545

Purpose

Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Lacra Bintu

Article

DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:

Insert

Surface marker and Citrine reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pVC228 VEGF Site#3

Plasmid

#47507

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 3 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-p27 T187A Luc

Plasmid

#23048

Depositor

William Kaelin

Article

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Tags

Luciferase

Expression

Mammalian

Mutation

T187A: mutation at codon 187 changing from T (ACG…

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArC Long WT

Plasmid

#59809

Purpose

Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-H169Q-GFP

Plasmid

#78917

Purpose

Mammalian expression of BAP1 with H169Q and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

H169Q

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTR AE Clover

Plasmid

#90240

Purpose

Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ErbB1(DEL1011)

Plasmid

#197366

Purpose

Encoding human ErbB1 mutant.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR mutant. (EGFR Human)

Expression

Mammalian

Mutation

deleted amino acids 1011-1186

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRVdGL-1Cre

Plasmid

#98038

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-siResist

Plasmid

#49852

Purpose

Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pRVdGL-4EGFP

Plasmid

#98037

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-C91G-GFP

Plasmid

#78916

Purpose

Mammalian expression of BAP1 with C91G and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

C91G

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660N

Plasmid

#45707

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660N

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pGEX6P1-Ubl-sr1(SIRPT1)

Plasmid

#75298

Purpose

Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 1-339

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ165

Plasmid

#109327

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Promoter

Promoter EC1.2 enhancer fused to EC1.1 promoter

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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