We narrowed to 9,520 results for: try

1. pGTM_COV2_NSP5_C145A_001_SUMO

   Plasmid

   #192349

   Purpose

   Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
   

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Cysteine 145 of the active site is substituted wi…

   Promoter

   T7 lac

   Available Since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pME GFP-PCNA

   Plasmid

   #105977

   Purpose

   entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

   Depositor

   Caren Norden

   Article

   Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

   Insert

   PCNA (PCNA Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV2/9-CW3SL-mCherry-DrTrkB

   Plasmid

   #184002

   Purpose

   AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkB (NTRK2 Human)
   

   Use

   AAV

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CAG-KKHSaCas9-BlastR

   Plasmid

   #107189

   Purpose

   Confers constitutive expression of KKH SaCas9.

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   KKH SaCas9 (NEWENTRY )
   

   Expression

   Bacterial

   Mutation

   E782K/N968K/R1015H

   Promoter

   Chicken ß-Actin

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

   Plasmid

   #136382

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-VPR
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

   Plasmid

   #194222

   Purpose

   Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   SynNotch-GAL4 DBD-VP64 AD
   

   Use

   Synthetic Biology

   Promoter

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. dpYPQ230-D156R-CBE1

   Plasmid

   #195367

   Purpose

   dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
   

   Use

   CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

   Tags

   3xflag

   Expression

   Plant

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-hs_ICAM_full (JDW 1047)

   Plasmid

   #224479

   Purpose

   Gateway compatible 5' entry clone containing the Human ICAM2 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   ICAM2 promoter
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. amilGFP chromoprotein promoter-less

    Plasmid

    #118006

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTarget TMEM97-P2A-mCardinal

    Plasmid

    #157747

    Purpose

    Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

    Depositor

    Andrew Kruse

    Article

    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

    Insert

    TMEM97 (TMEM97 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. p221z-erVenusYFP

    Plasmid

    #71265

    Purpose

    Entry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    VenusYFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p221z-erTagRFP

    Plasmid

    #71266

    Purpose

    Entry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    TagRFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. DT049A

    Plasmid

    #159410

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    tet promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p221k-erTq2CFP

    Plasmid

    #71264

    Purpose

    Entry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Tq2CFP
    

    Use

    Gateway

    Tags

    ER retention signal HDEL and signal peptide (ER)…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR4 TNRC6A

    Plasmid

    #20021

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Trinucleotide repeat containing 6A (TNRC6A Human)
    

    Use

    Entry vector

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBlastR-12xLexAOp-mCherry-MiniWhite

    Plasmid

    #165917

    Purpose

    Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Htr5b(3.7)-EGFP-sv40

    Plasmid

    #184411

    Purpose

    AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Mouse Htr5b gene

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR P2R-P3-ECFP

    Plasmid

    #48353

    Purpose

    Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Mutation

    Contains a stop codon.

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. tsPurple chromoprotein promoter-less

    Plasmid

    #118007

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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