We narrowed to 14,580 results for: pET

1. pET15b I-SceI (Ctermhis) K223I

   Plasmid

   #195615

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C2694G, G2345C, G2351C, A3365T, A3366T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b I-SceI (Ctermhis) K122I

   Plasmid

   #195614

   Purpose

   Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity

   Depositor

   Frederick Gimble

   Article

   Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

   Insert

   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C2458G, A2826T, A2827T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET23PI-Sce ESARC D218A

   Plasmid

   #195608

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T928C, T929C, A942C

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET PI-SceI C-His K301A

   Plasmid

   #195611

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

   Depositor

   Frederick Gimble

   Article

   He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A822G, A823C, A824T

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM-60-NcQDE2_506-938-H609A_O

   Plasmid

   #147083

   Purpose

   Bacterial Expression of NcQde-2_506-938-H609A

   Depositor

   Elisa Izaurralde

   Insert

   NcQde-2_506-938-H609A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETM-60-NcQDE2_506-938-T642E_O

   Plasmid

   #147084

   Purpose

   Bacterial Expression of NcQde-2_506-938-T642E

   Depositor

   Elisa Izaurralde

   Insert

   NcQde-2_506-938-T642E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETM-41P-DmGW182_635-861_N

   Plasmid

   #147014

   Purpose

   Bacterial Expression of DmGW182_635-861

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_635-861 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETM-41P-DmGW182_700-840_N

   Plasmid

   #147015

   Purpose

   Bacterial Expression of DmGW182_700-840

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_700-840 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETM-41P-DmGW182_700-861_N

   Plasmid

   #147016

   Purpose

   Bacterial Expression of DmGW182_700-861

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_700-861 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM-41P-DmGW182_744-840_N

    Plasmid

    #147017

    Purpose

    Bacterial Expression of DmGW182_744-840

    Depositor

    Elisa Izaurralde

    Insert

    DmGW182_744-840 (gw Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    One non silent mutation N799S and one silent muta…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge