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SIV URA PCP-NLS-GFPEnvy

Plasmid

#189941

Purpose

Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Article

Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

Insert

PCP-NLS-GFPEnvy

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDEINO4

Plasmid

#179488

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aadA1

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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S3_001_026_MV2

Plasmid

#134422

Purpose

pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Use

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Plasmid

#58440

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pDE43-MCK

Plasmid

#49523

Purpose

Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Use

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo6

Plasmid

#203957

Purpose

Cloning plasmid containing the kanamycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Kanamycin Resistance Gene

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Endoplasmic reticulum-targeted optodPLD

Plasmid

#140059

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CIBN, CRY2, P2A, Sac1(TM), and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJES103

Plasmid

#135410

Purpose

E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter

Depositor

Miriam Braunstein

Article

McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9

Insert

blaTEM-1

Use

Tags

Expression

Bacterial

Mutation

E. coli 'blaTEM-1 with M. tuberculosis mpt63…

Promoter

hsp60

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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RP1B-PP1alpha

Plasmid

#51768

Purpose

Expresses PP1 alpha residues 7-330

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

pp1 alpha

Use

Tags

His, TEV protease site, and Thio-6

Expression

Bacterial

Mutation

Contains PP1 alpha residues 7-330

Promoter

T7-lac

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Use

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-mVenus-I

Plasmid

#163763

Purpose

Expresses Streptomyces codon-optimised mVenus-I

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

mVenus-I

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

SP44

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer2:sHyper2

Plasmid

#84737

Purpose

Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Use

Tags

NLS-SV40

Expression

Plant

Mutation

Promoter

pFMV/p35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Spiralin

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pErbB2-ECFP

Plasmid

#66945

Purpose

Mammalian expression of rat ErbB2 tagged with ECFP

Depositor

Bo van_Deurs, Mads Lerdrup

Article

Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

Insert

ErbB2 (Erbb2 Rat)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
attB53-Pac-attB53

Plasmid

#183610

Purpose

RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

Pac

Use

Rmce

Tags

Expression

Mutation

Promoter

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo10

Plasmid

#203961

Purpose

Cloning plasmid containing the erythromycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Erythromycin Resistance Gene

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGH156_MS2-AID-Hygro

Plasmid

#85415

Purpose

lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID and Hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMT85_P438-mEOS3.2_GentaR

Plasmid

#173894

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

P438

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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