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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,628 results for: Nes

Showing: 6621 - 6640 of 24628 results

  1. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

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  2. pUC57-BBclone1

    Plasmid
    #137069
    Purpose
    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Inserts
    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    Use
    Tags
    Expression
    Bacterial
    Mutation
    wild-type, full-length (signal-peptide-containing…
    Promoter
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137069-plasmid-map-sequence-id-275695

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  3. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

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  4. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103869-plasmid-map-sequence-id-199177

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  5. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Use
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  6. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

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  7. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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  9. pSNAPf-N1

    Plasmid
    #58187
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58187-plasmid-map-sequence-id-475530

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  10. pSNAPf-C1

    Plasmid
    #58186
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58186-plasmid-map-sequence-id-314094

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  11. pMOTag2T-crelox-PUR

    Plasmid
    #24026
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24026-plasmid-map-sequence-id-11297

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  12. pMOTag2T-crelox-HYG

    Plasmid
    #24025
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    24025-plasmid-map-sequence-id-11296

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  13. pMOTag4H-crelox-HYG

    Plasmid
    #24027
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    3XHA
    Expression
    Mutation
    Promoter
    Available Since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24027-plasmid-map-sequence-id-11298

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  14. pMOTag4H-crelox-PUR

    Plasmid
    #24028
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    3XHA
    Expression
    Mutation
    Promoter
    Available Since
    June 21, 2010
    Availability
    Academic Institutions and Nonprofits only
    24028-plasmid-map-sequence-id-11299

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  15. pMOTag43M-crelox-PUR

    Plasmid
    #24032
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    3XHA
    Expression
    Mutation
    Promoter
    Available Since
    June 23, 2010
    Availability
    Academic Institutions and Nonprofits only
    24032-plasmid-map-sequence-id-11303

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  16. pMOTag43M-crelox-HYG

    Plasmid
    #24031
    Depositor
    George Cross
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    3XMyc
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    24031-plasmid-map-sequence-id-11302

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  17. pXRDRE-hyg

    Plasmid
    #31269
    Depositor
    Andrew Pierce
    Insert
    XRDRE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 20, 2011
    Availability
    Academic Institutions and Nonprofits only
    31269-plasmid-map-sequence-id-14332

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  18. pGCGFP-puro

    Plasmid
    #31266
    Depositor
    Andrew Pierce
    Insert
    GCGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    31266-plasmid-map-sequence-id-14329

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  19. pGCGFP-blcd

    Plasmid
    #31263
    Depositor
    Andrew Pierce
    Insert
    GCGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    31263-plasmid-map-sequence-id-14322

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  20. pGCGFP-hyg

    Plasmid
    #31265
    Depositor
    Andrew Pierce
    Insert
    GCGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    31265-plasmid-map-sequence-id-14328

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Showing: 6621 - 6640 of 24628 results