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PF13_0125-bio-His

Plasmid

#50808

Purpose

Expresses enzymatically monobiotinylated full length PF13_0125

Depositor

Gavin Wright

Publication

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF13_0125

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF14_0044-bio-His

Plasmid

#50809

Purpose

Expresses enzymatically monobiotinylated full length PF14_0044

Depositor

Gavin Wright

Publication

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF14_0044

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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AU1-TTC30A1-pcDNA3

Plasmid

#47323

Purpose

Expresses AU1 epitope-tagged TTC30A1 in mammalian cells

Depositor

Richard Maurer

Publication

Howard et al Exp Cell Res. 2013 Jun 25. pii: S0014-48

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TTC30A1 (Ttc30a1 Mouse)

Tags

AU1 epitope

Expression

Mammalian

Promoter

CMV

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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GST-FUS ΔNLS 1-513

Plasmid

#44984

Depositor

Robin Reed

Publication

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS ΔNLS (1-513aa) (FUS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 1-513

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pET16b-Trx80

Plasmid

#36945

Depositor

Anna Blom

Publication

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Trx80 (truncated Trx) (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

premature stop inserted after K80 in Trx1 gene

Promoter

T7

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
HL 3904 (=HL 770 + pHL 991)

Plasmid

#30093

Depositor

Han Lim

Publication

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJB3

Plasmid

#19552

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3 Mouse)

Tags

His

Expression

Mammalian

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pGEM-rat Fcepsilon RI gamma

Plasmid

#8375

Depositor

Jean Kinet

Publication

Blank et al Nature 1989 Jan 12;337(6203):187-9.

Insert

rat Fc epsilon RI gamma cDNA (Fcer1g Rat)

Use

Beta-gal

Expression

Bacterial

Available since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
GPR77-DuET

Plasmid

#213291

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPR77 (C5AR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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mito-PDCD10-mScarlet-I

Plasmid

#208887

Purpose

Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Publication

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM3 (PDCD10 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

PDCD10 targeted to the mitonchondria

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-HseIF4E_AF

Plasmid

#148669

Purpose

Bacterial Expression of HseIF4E

Depositor

Elisa Izaurralde

Publication

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E (EIF4E Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence of i…

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mGas8-PA

Plasmid

#176468

Purpose

Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Publication

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

growth arrest specific 8 (Gas8 Mouse)

Tags

PA

Expression

Mammalian

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Publication

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE052 ZF43x3-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138733

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE054 ZF43x12-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138735

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_ARP3B

Plasmid

#111680

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ARP3B (ACTR3B Human)

Expression

Mammalian

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_TR61B

Plasmid

#111679

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TR61B (TRMT61B Human)

Expression

Mammalian

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBRN168-DXMT

Plasmid

#79821

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaDXMT1

Use

Reporter plasmid

Tags

RN168/DsREDmonomer (1–168)

Expression

Plant

Promoter

CaMV35S and CaMV 35S promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBCN155-MXMT

Plasmid

#79815

Purpose

CFP-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

CN155/CFP (1–155)

Expression

Plant

Promoter

CaMV35S and CaMV 35S promoter

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBGN155-MXMT

Plasmid

#79817

Purpose

GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

GN155/GFP (1–155)

Expression

Plant

Promoter

CaMV35S and CaMV 35S promoter

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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