We narrowed to 9,085 results for: mel

1. JAK2 (V617F)-pcw107-V5

   Plasmid

   #64610

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   V617F

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-hygro-2xMyc-Rac1-P29S

   Plasmid

   #128581

   Purpose

   For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Proline29 to Serine.

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-hygro-2xMyc-Rac1-Q61L

   Plasmid

   #128582

   Purpose

   For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Glutamine61 to Leucine.

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-F-Med26-NTD R61A K62A

   Plasmid

   #49256

   Purpose

   Mammalian expression of flag-tagged human Med26-NTD R61A K62A

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   NTD R61A K62A

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGEX6P2(GST-SNAP-Shp2tSH2)

   Plasmid

   #177907

   Purpose

   Baculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domains

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   Shp2 tandem SH2 domains (aa 6-218 only) (PTPN11 Human)
   

   Tags

   GST-PreScission cut site-SNAP tag

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-TetO-lox-ERAS

   Plasmid

   #52417

   Purpose

   Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   ERAS (ERAS Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. INCTbiosyn-p35S(rc)2_4_5-GFP

   Plasmid

   #127520

   Purpose

   Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEANHISECArdB

   Plasmid

   #96967

   Purpose

   Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   E. coli ArdB
   

   Tags

   N-ter TEV cleavable 6His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α-FLEX-rc[Chronos-GFP]

   Plasmid

   #62725

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

    Plasmid

    #157744

    Purpose

    knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TGFbetaR1 (T204D)-pcw107-V5

    Plasmid

    #64629

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    TGFBR1 (transcript variant 1) (TGFBR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    T204D

    Promoter

    PGK

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

12. INCTbiosyn-pEF-GFP(rc)Bxb1

    Plasmid

    #127511

    Purpose

    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT/T0-Flag-RECQL5

    Plasmid

    #222620

    Purpose

    Mammalian expression vector of flag-tagged RECQL5

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    RECQL5 (RECQL5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. INCTbiosyn-pEF-GFP(rc)phiC31

    Plasmid

    #127510

    Purpose

    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    

    Expression

    Mammalian

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84484

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe.puro.CDK8.flag

    Plasmid

    #19758

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3TS-zSpG

    Plasmid

    #179316

    Purpose

    In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Zebrafish codon-optimized Cas9 variant SpG
    

    Use

    CRISPR; In vitro transcription by t3 promoter

    Tags

    SV40-NLS

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3TS-zSpRY

    Plasmid

    #179317

    Purpose

    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Zebrafish codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR; In vitro transcription by t3 promoter

    Tags

    SV40-NLS

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-SpG-His

    Plasmid

    #179318

    Purpose

    Plasmid for bacterial expression and purification of SpG-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpG
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Notch3 intracellular domain-pcw107-V5

    Plasmid

    #64623

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH3 (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only