We narrowed to 9,109 results for: control

1. pgRNA-non-target

   Plasmid

   #129465

   Purpose

   Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Non-target/random gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 (SpeI)

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBH547_Tet-Cas14b2Locus

   Plasmid

   #112504

   Purpose

   Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS423-TyrSgH

   Plasmid

   #163973

   Purpose

   Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-TK-Firefly-cjBCCIP

   Plasmid

   #122278

   Purpose

   Lentiviral vector for stable expression of Firefly luciferase.

   Depositor

   Demián Cazalla

   Article

   Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

   Inserts

   Firefly luciferase
   cjBCCIP 3'UTR
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBrain-GFP-shGL2

   Plasmid

   #60004

   Purpose

   Co-expresses control GL2 shRNA and GFP.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   EGFP
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA-FRT-FAK-kinase30

   Plasmid

   #59124

   Purpose

   FAK fret control sensor without FERM domain with 30 nm linker

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. TET-pLKO.1 PURO shGFP

   Plasmid

   #83085

   Purpose

   Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

8. CD200Cd4d3+4-bio

   Plasmid

   #32404

   Purpose

   A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

   Depositor

   Gavin Wright

   Article

   Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

   Insert

   CD200
   

   Use

   Tags

   Cd4d3+4 and biotinylation peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAC690-TEF

   Plasmid

   #160432

   Purpose

   Expresses eGFP under control of the yeast TEF2 promoter

   Depositor

   Gary Stormo

   Article

   Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

   Insert

   S. cerevisiae TEF2 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   S. cerevisiae TEF2

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. DT042A

    Plasmid

    #159403

    Purpose

    Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.T5-LacO-6xHis-sfGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TET-pLKO.1 PURO shLacZ

    Plasmid

    #98632

    Purpose

    Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLacZ
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD200Cd4d3+4-blac

    Plasmid

    #32405

    Purpose

    A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    CD200
    

    Use

    Tags

    Cd4d3+4 and beta-lactamase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT11F

    Plasmid

    #165062

    Purpose

    Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPMVAugw

    Plasmid

    #98300

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TEFM1EfmvaE>TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-NBid-mMaple-CBid

    Plasmid

    #164049

    Purpose

    Optogenetic manipulation of cell apoptosis negative control

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-mMaple-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid

    #68405

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Synthetic Probasin ARRx2 and U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CBla_2.0_EmrE_TMD4_3P

    Plasmid

    #207848

    Purpose

    BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

    Depositor

    Dieter Langosch

    Article

    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

    Insert

    C-BLa 2.0 fusion protein
    

    Use

    Tags

    Flag (DYKDHDG)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH613

    Plasmid

    #169602

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB1H2w2-12-Oct1

    Plasmid

    #18041

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    HD of Oct1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the homeodomain of Oct1 is fused to fingers 1 and…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-P70a-Top-T~P70a-Bottom-T

    Plasmid

    #87314

    Purpose

    Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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