We narrowed to 7,672 results for: CCH

1. pRS413-TEF1pr-SEC7-FAPoptim

   Plasmid

   #221124

   Purpose

   FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   SEC7-FAPoptim
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS413-TEF1pr-VPH1-FAPoptim

   Plasmid

   #221125

   Purpose

   FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   VPH1-FAPoptim
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCY5k

   Plasmid

   #160294

   Purpose

   Yeast CRISPR plasmid targeting the kanMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB8156

   Plasmid

   #175200

   Purpose

   Xenopus oocyte expression vector containing yEGFP. Contains T7 RNA polymerase binding site, and adds polyA tail to yEGFP. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   yEGFP
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Promoter

   T7

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEndo-I-OnuI-TSM

   Plasmid

   #207954

   Purpose

   Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.

   Depositor

   David Edgell

   Article

   Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

   Insert

   Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Leucine 212 changed to proline, leucine 434 in fu…

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHis-WzzE

   Plasmid

   #196661

   Purpose

   Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzzE
   

   Tags

   TEV-cleavable His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGST-WzzE

   Plasmid

   #196662

   Purpose

   Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzzE
   

   Tags

   TEV-cleavable GST

   Expression

   Bacterial

   Mutation

   Valine insertion at position 2.

   Promoter

   T7

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHis-mCherry-WzzE

   Plasmid

   #196663

   Purpose

   Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzzE
   

   Tags

   His and mCherry

   Expression

   Bacterial

   Mutation

   Valine insertion at position 2 of WzzE.

   Promoter

   T7

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPglL-strep-mCherry

   Plasmid

   #196664

   Purpose

   Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   PglL
   

   Tags

   TEV-cleavable distrep tag II and mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPglL-strep-mClover3

    Plasmid

    #196665

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWzyE-GST-distrepII

    Plasmid

    #196666

    Purpose

    Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Tags

    GST and TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWzyE-strep-mClover3

    Plasmid

    #196668

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFPEasy9R

    Plasmid

    #218291

    Purpose

    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pToxAmp21R

    Plasmid

    #218287

    Purpose

    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pToxAmp12

    Plasmid

    #218286

    Purpose

    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pToxAmp1

    Plasmid

    #218285

    Purpose

    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pILGFP4QE2Crimson

    Plasmid

    #218280

    Purpose

    Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKG-GW1-SRT1

    Plasmid

    #203171

    Purpose

    Yeast expression vector for yeast SRT1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    SRT1 (SRT1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG

    Plasmid

    #212809

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Tags

    NUbo and mCherry-FLAG

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

    Plasmid

    #212804

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-P2A-3xFLAG-Ubc7
    

    Expression

    Mammalian

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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