We narrowed to 26,139 results for: Vit

1. pSG058

   Plasmid

   #214261

   Purpose

   PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. WN10151

   Plasmid

   #80441

   Purpose

   Expresses human codon-optimized dead (832A) LbCpf1

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Insert

   dead (D832A) LbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NG-ABE8-dimer

   Plasmid

   #138497

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. ∆DUSP6

   Plasmid

   #27977

   Depositor

   Igor Astsaturov

   Article

   Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

   Insert

   DUSP6 (DUSP6 Human)
   

   Use

   Lentiviral

   Tags

   HA and MYC

   Expression

   Mammalian

   Mutation

   C293S: mutation in the phosphatase domain

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-MBII85-5'SS mut

   Plasmid

   #67631

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutation in 5'ss

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. SlugCas9-HF

   Plasmid

   #163796

   Purpose

   Expresses highly specific SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   SlugCas9 (R247A, N415A, T421A, R656A)

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-ChroME-mRuby2-ST

   Plasmid

   #108902

   Purpose

   Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChroME-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC22A2

   Plasmid

   #131964

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A2 (SLC22A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti-FNLSNG-P2A-Puro

   Plasmid

   #136900

   Purpose

   Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSynapsin1-axon-GCaMP6s

    Plasmid

    #111262

    Purpose

    For enriched expression of GCaMP6s in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV1

    Insert

    axon-GCaMP6s
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. eNme2-C TadCBEd

    Plasmid

    #193841

    Purpose

    Express TadCBEd (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadCBEd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mas-GRK2RH-Nluc

    Plasmid

    #158605

    Purpose

    Plasmid expressing GRK2(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMPMA3dPlac-PprgH-GFPLVA

    Plasmid

    #23340

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pVSL10

    Plasmid

    #201182

    Purpose

    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

    Depositor

    Jack Szostak

    Article

    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

    Insert

    Bst polymerase large fragment (BF) F710Y/D598A
    

    Use

    Tags

    HRV 3C protease cleavage site and His6

    Expression

    Bacterial

    Mutation

    F710Y D598A

    Promoter

    T5-lacO

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-fSST-RFP

    Plasmid

    #22913

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    fugu somatostatin promoter driving DsRed 2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+ Lifeact-mCherry

    Plasmid

    #185408

    Purpose

    Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    lifeact
    

    Use

    In vitro synthesis of mrna

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. C3 luc (T1 del)

    Plasmid

    #11358

    Depositor

    Donald McDonnell

    Article

    Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

    Insert

    C3 promoter (C3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-hDicer

    Plasmid

    #89144

    Purpose

    Expression of hDicer in Sf9 Cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    hDicer (DICER1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTrcHisB-TRF2

    Plasmid

    #50488

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 (TERF2 Human)
    

    Use

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-500

    Promoter

    pTrc

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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