We narrowed to 7,531 results for: Ski;

1. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

   Plasmid

   #197508

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

   Promoter

   CMV and T7

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_MAX_WT_V5

   Plasmid

   #82944

   Purpose

   Gateway Donor vector containing MAX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   MAX (MAX Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLD-puro-Cc-TARDBP-WT_VA

   Plasmid

   #141327

   Purpose

   Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

   Depositor

   Mohan Babu

   Article

   Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

   Insert

   TARDBP-WT (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

   Expression

   Mammalian

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.3_SARS2_XBB

   Plasmid

   #194494

   Purpose

   expressing SARS-CoV-2 XBB spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

   Insert

   Spike (XBB) (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

   Promoter

   CMV

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.3_SARS2_BQ.1.1

   Plasmid

   #194493

   Purpose

   expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

   Insert

   Spike (BQ.1.1) (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   R346T, K444T, N460K and c-terminal 18aa del on th…

   Promoter

   CMV

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5-mEGFP-MYOD1-WT

   Plasmid

   #194570

   Purpose

   Mammalian Expression of mEGFP-MYOD1 WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   MYOD1 (MYOD1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223_CFLAR_WT_V5

   Plasmid

   #82936

   Purpose

   Gateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CFLAR (CFLAR Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_KRAS_p.G13D

   Plasmid

   #82808

   Purpose

   Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   G13D

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_KRAS_p.G12R

   Plasmid

   #82804

   Purpose

   Gateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   G12R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_ERBB2_p.S310F

    Plasmid

    #82854

    Purpose

    Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, Jesse Boehm, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Gateway entry vector

    Mutation

    S310F

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCXLE-SOX2AV-p2a-KLF4

    Plasmid

    #193291

    Purpose

    episomal expressing human SOX2A61V + KLF4

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    SOX2AV-p2a-KLF4 (SOX2, KLF4 Human)
    

    Expression

    Mammalian

    Mutation

    SOX2A61V is a highly cooperative point mutant of …

    Promoter

    CAG

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRIPZ-p21-HA(RxL)

    Plasmid

    #215111

    Purpose

    Expresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

    Promoter

    CMV (Tet inducible)

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCXLE-mCherry-t2a-SOX2-p2a-KLF4

    Plasmid

    #193296

    Purpose

    episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-KrasG12D/sgKras/Cre

    Plasmid

    #99851

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid

    #208293

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…

    Promoter

    CMV and T7

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid

    #208290

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations and V106W in TadA and nSpCas9(D10…

    Promoter

    CMV and T7

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.3_SARS2_BA.4/5

    Plasmid

    #186810

    Purpose

    pseudovirus plasmid expressing BA.4 and BA.5 spike protein

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    43740568 (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    HY69-70del, L452R, F486V, Q493R, on the basis of …

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_EBNA1BP2_WT_V5

    Plasmid

    #82932

    Purpose

    Gateway Donor vector containing EBNA1BP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, Jesse Boehm, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EBNA1BP2 (EBNA1BP2 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-45b-mCherry-Cbx5

    Plasmid

    #194547

    Purpose

    Bacterial Expression of 6xHis mCherry-HP1a Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    Cbx9 (HP1a)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-mEGFP-PHOX2B-WT

    Plasmid

    #194576

    Purpose

    Mammalian Expression of mEGFP-PHOX2B WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    PHOX2B (PHOX2B Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge