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pCINeo-hFX(I154F)-HA

Plasmid

#14977

Depositor

Michael Ristow

Article

Schulz et al J Biol Chem. 2006 Jan 13. 281(2):977-81.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Mutation

changed isoleucine 154 to phenylalanine

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
GFP-RanV19 E1RA2

Plasmid

#118317

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Inserts

RanV19 (RAN Human)
EPac1RA2 (RAPGEF3, RAPGEF4 Human)

Tags

GFP

Expression

Mammalian

Mutation

in-frame insertion of RanV19 into GFPEpac1RA2 and…

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1Δ∼PRD

Plasmid

#71727

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Budding Yeast, Herpes Simplex Virus protein VP16, Human)

Expression

Mammalian

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/VSVG Δ67a

Plasmid

#253172

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD2 ΔE6 3* Δr7a/VSVG

Plasmid

#253171

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a (residual AA);stop codon at AA213 in orf3a; VSV glycoprotein replaces orf7a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with VSV-G

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pX330_H1.2

Plasmid

#247480

Purpose

Expresses SpCas9 and a sgRNA targeting the human histone H1.2 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.0_HaloTag

Plasmid

#247483

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.0 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.0 (H1F0) (H1-0 Human)

Use

CRISPR

Tags

HaloTag

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.2_mAID_mClover

Plasmid

#247486

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.2 with mAID-mClover

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Use

CRISPR

Tags

mAID and mClover

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01701_CMV-rtTA-betaglobin_pA-CMV-PuroR-bGH

Plasmid

#252540

Purpose

Secondary PiggyBac vector for puromycin selection and expression of transcriptional activator

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

rtTA

Expression

Mammalian

Promoter

CMV (human cytomegalovirus immediate early enhanc…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01416_TRE-TagBFP-bGH-EF1alpha-mRuby2-SV40

Plasmid

#252541

Purpose

Inducible gene and constitutive reporter with downstream tandem syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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