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Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB6
Plasmid#83557PurposeUsed to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.DepositorInsertGAL10 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq20-T38S38-0X
Plasmid#49522PurposeKanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertconstitutive TetR38
UseTagsExpressionBacterialMutationPromoterPtb38Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dom34(1-377)
Plasmid#19100DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E23K
Plasmid#19105DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE23KPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E149K
Plasmid#19107DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE149KPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 F218A
Plasmid#19108DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationF218APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E261K
Plasmid#19109DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE261KPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 Y300A
Plasmid#19110DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationY300APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 K45A
Plasmid#19111DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationK45APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCpSRb-5
Plasmid#13727DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHisExpressionBacterialMutationSRβ circularly permuted D210E188 improved solubil…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorUseTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…PromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-PEmax-P2A-Puro
Plasmid#228890PurposeLentiviral delivery and expression of PEmax protein with puromycin selection cassette.DepositorInsertsPEmax
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-HygR-mCherry
Plasmid#167189PurposeLentiviral expression of sgRNA with hygromycin resistance gene and mCherryDepositorInsertHygromycin B phosphotransferase and mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
HDAC5 Flag
Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorInsertHDAC5 (HDAC5 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Hph
Plasmid#188741PurposepUC19 plasmid (MCS intact) with Hygromycin selection markerDepositorInsertHygromycin
UseFilamentous fungal expressionTagsExpressionMutationPromotergpdAAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-12xHRE
Plasmid#172335Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only