We narrowed to 7,199 results for: RAP

1. pCMV-BE2

   Plasmid

   #73020

   Purpose

   Expresses BE2 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-SCYL1-V5-miniTurbo_IDG-K

   Plasmid

   #189911

   Purpose

   Gateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   SCYL1 (SCYL1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-X1-hygro-mCherry-RAMP4

   Plasmid

   #118391

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   RAMP4 (SERP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lys-eMagA-EGFP

   Plasmid

   #162246

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   p18-eMagA-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-BE1

   Plasmid

   #73018

   Purpose

   Expresses BE1 with N-terminal His6 tag in E. Coli

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-BICD2(1-500)-FRB

   Plasmid

   #174648

   Purpose

   Rapalog-inducible coupling to dynein adaptor BICD2

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   HA-BICD2(1-500)-FRB
   

   Use

   Tags

   FRB and HA

   Expression

   Mammalian

   Mutation

   mmBICD2(aa1-500): Lys213Glu

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5KO9 (HMCES)

   Plasmid

   #101877

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HMCES (HMCES Human)
   

   Use

   Tags

   6x His (modified C-term tag is AENLYFQSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCS2 Notch1 ICv-6MT

   Plasmid

   #41730

   Depositor

   Raphael Kopan

   Article

   Schroeter et al Nature. 1998 May 28;393(6683):382-6.

   Insert

   Notch-1 Intracellular Domain (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Contains murine Notch-1 protein beginning at Vali…

   Promoter

   Available since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMito-GBPen

   Plasmid

   #128269

   Purpose

   Expression of mitochondrially targeted GBPen (GFP Nanobody)

   Depositor

   Stephen Royle

   Article

   Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

   Insert

   Mito-GBPen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

    Plasmid

    #58380

    Purpose

    tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

    Depositor

    Didier Stainier

    Article

    Housley et al Development. 2014 Oct;141(20):3988-93. d

    Insert

    avi-EGFP-rpl10a
    

    Use

    Tags

    Avi Tag and EGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMito-PAGFP-FRB.

    Plasmid

    #60005

    Purpose

    Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Type

    Empty backbone

    Use

    Knocksideways

    Tags

    FRB and PAGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. FRB-polySH35

    Plasmid

    #175234

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FRB-polySH33

    Plasmid

    #175233

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-Nore1

    Plasmid

    #1979

    Depositor

    Joseph Avruch

    Article

    Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

    Insert

    Nore1 (Rassf5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

15. FLAG-Nore1

    Plasmid

    #1975

    Depositor

    Joseph Avruch

    Article

    Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

    Insert

    Nore1 (Rassf5 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pJD427

    Plasmid

    #154371

    Purpose

    Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

    Depositor

    Joseph Davis

    Article

    Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

    Insert

    proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GST-Nore1

    Plasmid

    #1971

    Depositor

    Joseph Avruch

    Article

    Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

    Insert

    Nore1 (Rassf5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

18. MZB216

    Plasmid

    #221766

    Purpose

    "Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    rtxA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SH4-FRB-EBFP2

    Plasmid

    #222418

    Purpose

    To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    SH4
    

    Use

    Tags

    FRB-HA-EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFOSGE_CUS1B08

    Plasmid

    #71087

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    MED7 (MED7 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only