We narrowed to 16,181 results for: nes
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Plasmid#50003PurposepUC cloning vectorDepositorPublicationTypeEmpty backboneExpressionBacterialPromoterlacAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only
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mApple-Display-5
Plasmid#54543PurposeLocalization: Cytoplasm, Excitation: 568, Emission: 592DepositorTypeEmpty backboneTagsmApple-DisplayExpressionMammalianAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMartini Gate B R2-R1
Plasmid#36434DepositorInsertGateway Cassette
UseGateway conversion vectorAvailable sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196082Purpose(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-SpdCas9-W3SL
Plasmid#210708PurposeThis Plasmid express hSyn promoter driven SpdCas9DepositorInsertS. Pyogenes dCas9
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationD10A/H840APromoterhSynAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x3
Plasmid#63597PurposeExpresses dCas9 and gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-7CtoA-LIN-41
Plasmid#52726Purposeretroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged cysteines 12, 15, 61, 66, 69, 91, and 94 …Available sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX3-C Cloning Vector
Plasmid#220615PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)DepositorTypeEmpty backboneTagsFlag-APEX3 fusionExpressionMammalianAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2
Plasmid#199481PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2
Plasmid#199482PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMaCTag-34
Plasmid#119998PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEx_Hsp70::EGFPpA-ttBtagRFPt
Plasmid#48877PurposePhiC31 exchange vector with the Hsp70::EGFPpA insert. Selection via EGFP to tagRFPt-color switch in the heartDepositorPublicationInsertHSP70::EGFPpA
Available sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-APOBEC1
Plasmid#209039PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLSDepositorInsertAPOBEC1-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)
Plasmid#179008PurposeSp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)
Plasmid#179002PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -