We narrowed to 2,455 results for: GEM

1. pGEMHE-MmCENPT-MsHFD-GFP

   Plasmid

   #237427

   Purpose

   Expresses Mus musculus CENP-T with Mus spretus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with Mus spretus HFD (Cenpt Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   HFD (residues 413-503) swapped from Mus spretus

   Promoter

   T7

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. EP003: CAG::GEM7-HaloTag

   Plasmid

   #209303

   Purpose

   Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation

   Depositor

   Adam Cohen

   Insert

   GEM7
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. p49.unipolyA.Cdt1.mSca.mCMV.PGK.eGFP.Gem1.WPRE

   Plasmid

   #226768

   Purpose

   vector with mScarlet fluorescent protein and GFP

   Depositor

   Ferdinando Pucci

   Article

   Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

   Plasmid

   #225436

   Purpose

   BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

   Depositor

   Diego Orzaez

   Insert

   BeYDV geminino 1.0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE:mTREK-1(tandem S131C-S131C)

   Plasmid

   #224760

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C / S562C

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMHE:mTREK-1 (S131A/C159A/C219A)

   Plasmid

   #224753

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131A/C159A/C219A

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE:mTREK-1 (S131C/C159A/C219A)

   Plasmid

   #224752

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C/C159A/C219A

   Promoter

   T7

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   601 has an additional mutation added 22 bp from d…

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLeo-A-GEMS(JAK-STAT)

   Plasmid

   #209109

   Purpose

   Transient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   A-GEMS(JAK-STAT)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLeo-A-a3-GEMS(JAK-STAT)

    Plasmid

    #209111

    Purpose

    Transient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    A-a3-GEMS(JAK-STAT)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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