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VPS13D1-1576-(Y768A)

Plasmid

#194006

Purpose

Y to A mutation in VPS13D gene

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D (VPS13D Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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VPS13D1-1576-(Y1253S)

Plasmid

#194005

Purpose

Y to S mutation in VPS13D gene

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D (VPS13D Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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VPS13D(∆ATG2-C/PH)^EGFP

Plasmid

#194004

Purpose

Deletion of DHPH domain of VPS13D

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D (VPS13D Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

early stop codon before DHPH

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-Miro1(∆TM)-SspB

Plasmid

#194007

Purpose

Optogenetic tool

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-HA

Plasmid

#180145

Purpose

Coding sequence of human FAM3B pCS2 with C terminal HA tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Bighead S-FC

Plasmid

#180147

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

Tags

FC

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-FLAG

Plasmid

#180146

Purpose

Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 Bighead S-HA

Plasmid

#172485

Purpose

for synthesis of mRNA for injection into xenopus laevis embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

In vitro mrna synthesis

Tags

HA and S-HA

Expression

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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CBSH3+ shRNA-2

Plasmid

#160068

Purpose

Knock Down SH3+ Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3+ 3m shRNA-1

Plasmid

#160067

Purpose

3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

Promoter

mouse U6 promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3+ shRNA-1

Plasmid

#160066

Purpose

Knock Down SH3+ Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA3

Plasmid

#160213

Purpose

Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

Promoter

mouse U6 promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- shRNA2

Plasmid

#160210

Purpose

Knock Down MTLSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Human, Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CBSH3- shRNA3

Plasmid

#160212

Purpose

Knock Down MYDSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Human, Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA2

Plasmid

#160211

Purpose

Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA1

Plasmid

#160209

Purpose

Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CBSH3- shRNA1

Plasmid

#160208

Purpose

Knock Down MQWSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Human, Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

Plasmid

#160069

Purpose

Conditionally overexpress collybistin (CB2SH3-) and mCitrine

Depositor

Angel de Blas

Article

George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

AAV

Tags

HA- in CB2SH3

Expression

Mutation

Promoter

human Synapsin promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Hu_TTK1(WT)

Plasmid

#136354

Purpose

Human TTK (Mps1) cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer

Insert

TTK (TTK Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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