Addgene

We narrowed to 976 results for: pegfp n1

Showing: 661 - 680 of 976 results

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NLS-dCas9-HA-NLS-GFP-VP16

Plasmid

#183923

Purpose

VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-VP16

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFR-PATagRFP

Plasmid

#31950

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Use

Tags

EGF receptor (EGFR)

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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TrkB mEGFP

Plasmid

#83952

Purpose

FRET donor for dynamically measuring TrkB activity in neurons

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Tropomyosin related kinase B (Ntrk2 Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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FoxO1-ADA-GFP

Plasmid

#35640

Depositor

Domenico Accili

Article

Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-4

Insert

FoxO1-ADA-GFP (Foxo1 Mouse)

Use

Tags

Flag, GFP, and Myc

Expression

Mammalian

Mutation

T24A, S253D and S316A mutations

Promoter

CMV

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pADSL-EGFP

Plasmid

#99109

Purpose

Expresses human ADSL-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

ADSL (ADSL Human)

Use

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRIP3-mCherry

Plasmid

#61386

Purpose

Expresses RIP3-mCherry in mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

RIP3 (RIPK3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
NLS-dCas9-HA-NLS--GFP-p300

Plasmid

#183925

Purpose

p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-p300

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-GalT

Plasmid

#11929

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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BICDN-CIBN

Plasmid

#102253

Purpose

Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

BICDN-CIB1 (CIB1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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ChR2-2A-Halo

Plasmid

#22047

Purpose

Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

Depositor

Edward Boyden

Article

Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

Insert

ChR2-GFP-2A-Halo-YFP

Use

Tags

GFP and YFP

Expression

Mammalian

Mutation

Note that there is a S147R mut in YFP. This mutat…

Promoter

CMV

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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NPY-mCherry

Plasmid

#67156

Purpose

Expresses NPY-mCherry

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

NPYmCherry (NPY Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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NPY-pHTomato

Plasmid

#83501

Purpose

Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

Depositor

Sebastian Barg

Article

Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

Insert

NPY (NPY Human)

Use

Tags

pHTomato

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Olof Idevall-Hagren, Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Use

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
PH-PLCD1(R40L)-GFP

Plasmid

#51408

Purpose

Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 40 mutated to leucine

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
PCDHGC5-EGFP

Plasmid

#119933

Purpose

Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPAGFP-VSVG

Plasmid

#11915

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Use

Tags

Expression

Mammalian

Mutation

(L64F/T65S/V163A/T203H). The PAGFP also contains …

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP2

Plasmid

#125250

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Use

Tags

Expression

Mammalian

Mutation

D396A

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Plasmid

#173870

Purpose

Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173872

Purpose

Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHJ423

Plasmid

#20681

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 567 to Asp

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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