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ASC-CASP1 Octamer
Plasmid#164032PurposeBacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamerDepositorUseTagsHis6-MBP-TEVExpressionBacterialMutationW169G (ASC), G20K (CASP1)PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmKO-imp α
Plasmid#200082PurposeExpresses mKO-tagged human importinα in mammalian cellsDepositorInsertimportin α (KPNA2 Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEL17
Plasmid#107923PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A
Plasmid#166801PurposeMammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.DepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationS730APromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE
Plasmid#166796PurposeMammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.DepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationKK93EEPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R
Plasmid#166800PurposeMammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.DepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationE597RPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-Hdm4-3'UTR-F2d5
Plasmid#64018PurposeLuciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5DepositorInsertHDMX (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1953
Plasmid#142897PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPARP1 (PARP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S RK96EE
Plasmid#166797PurposeMammalian cell expression vector for FLAG-tagged DPP9S. RK96EE mutant is partial loss of CARD8 binding.DepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationRK96EEPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL102EE
Plasmid#166799PurposeMammalian cell expression vector for FLAG-tagged DPP9S. LL102EE mutant is partial loss of CARD8 binding.DepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationLL102EEPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S D808N
Plasmid#166802PurposeMammalian cell expression vector for FLAG-tagged DPP9S. The D808N mutant is catalytically dead.DepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationD808NPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-imp α
Plasmid#119718PurposeExpresses EGFP-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
UseTagsEGFPExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-FBXL11
Plasmid#126543PurposeExpresses GFP-tagged FBXL11 in mammalian cellsDepositorInsertFBXL11 (KDM2A Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterLTRAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROS17
Plasmid#107931PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ABCF2_STOP
Plasmid#221425PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertABCF2 (ABCF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 blast OFF 3xflag IREB2
Plasmid#169889PurposeExpress 3x flag IREB2, suppressible by doxycycline additionDepositorInsertIREB2 (IREB2 Human)
UseLentiviral; Doxycycline mediated suppressionTags3x FlagExpressionMammalianMutationSilent mutations in sgIREB2_1 and sgIREB2_2 targe…PromoterTREAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-imp α
Plasmid#119719PurposeExpresses mCherry-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
UseTagsmCherryExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only
