We narrowed to 7,762 results for: loxP

Showing: 6781 - 6800 of 7762 results

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Publication

Björefeldt et al Neurophotonics. 2024 Apr;11(2):021005. d

Insert

mNeonGreen, eKL9h, and Volvox channelrhodopsin-1

Use

AAV

Promoter

hSyn

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1A

Plasmid

#198168

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(285)-GFP

Plasmid

#197889

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.0

Plasmid

#190795

Purpose

PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

Depositor

Omar Akbari

Publication

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

PRE-Hsp70Bb-Cas9-T2A-eGFP

Use

CRISPR

Expression

Insect

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2265

Plasmid

#175962

Purpose

For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Tags

8xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Δ347-361, replace with 702-716

Promoter

araBAD

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMA4000

Plasmid

#184857

Purpose

Cre-excisable TFAM in Blasticidin-resistannce retroviral vector

Depositor

Mikhail Alexeyev

Publication

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

TFAM (TFAM Human)

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTL464

Plasmid

#69881

Purpose

a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

Depositor

Robert Kittel, Tobias Langenhan

Publication

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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GSX2-P2A-tGFP-DTA

Plasmid

#161750

Purpose

tGFP plasmid for the c-terminal targeting of human GSX2 locus

Depositor

Elena Cattaneo

Publication

Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

Insert

turboGFP

Use

CRISPR

Tags

P2A-tGFP

Expression

Mammalian

Promoter

endogenous GSX2 promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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DHC1-mIAA17 Hygro

Plasmid

#140544

Purpose

DHC1 tagging with mIAA7

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050L (UASt-CasRx)

Plasmid

#132418

Purpose

Express CasRx under UASt promoter

Depositor

Omar Akbari

Publication

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

CasRx
UASt+hsp70

Expression

Insect

Promoter

UASt

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Publication

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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miR290-SE-RGM-tdTomato targeting vector

Plasmid

#133047

Purpose

Targeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMR

Depositor

Rudolf Jaenisch

Publication

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

miR290-SE-5arm-RGM-miR290-SE-3arm

Use

Mouse Targeting

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e5-S253T

Plasmid

#129755

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

S253T

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e5-P216S

Plasmid

#129754

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

P216S

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2_K58R_Keima

Plasmid

#127141

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2_Keima (RPS2 Human)

Use

Lentiviral

Mutation

K58R

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-attB V5 dDispatched delta CS HA

Plasmid

#126412

Purpose

Expression of V5 dDispatched Delta CS HA in Drosophila cells lines

Depositor

Stacey Ogden

Publication

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Mutation

Disp1 Cleavage site

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF8_P2A_Hygro_Barcode

Plasmid

#120494

Purpose

Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF8 (KLF8 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mTet1 flox donor

Plasmid

#117147

Purpose

Making Tet1 conditional knock out

Depositor

Jacob Hanna

Publication

Zviran et al ssrn.3155731

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pFL His-I3 V41A / W43A

Plasmid

#113515

Purpose

Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Tags

6*His

Expression

Insect

Mutation

V41A /W43A

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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