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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,076 results for: pcdna3.1

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Showing: 6801 - 6820 of 7076 results

  1. pcDNA3-mCherry-Rheb(5A)

    Plasmid
    #192439
    Purpose
    Encodes mCherry-tagged Rheb lacking residues 38-42.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Rheb(C181S) (RHEB Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Rheb amino acids 38-42 deleted.
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192439-plasmid-map-sequence-id-379136

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  2. pcDNA3-mCherry-TSC2-NLS

    Plasmid
    #192441
    Purpose
    Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192441-plasmid-map-sequence-id-379077

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  3. pCDZ2FE_Champ1_1-812_2PA

    Plasmid
    #105676
    Purpose
    Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P336A, P341A
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105676-plasmid-map-sequence-id-203181

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  5. pcDNA3-bio-myc-mCE 2-210

    Plasmid
    #112715
    Purpose
    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells
    Depositor
    Daniel Schoenberg
    Publication
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rngtt (Rngtt Mouse)
    Tags
    biotinylation signal peptide (Tagwerker et al. 20…
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    112715-plasmid-map-sequence-id-217167

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  6. FLAG-MBP1-E322TAG-W340A.pcDNA3-k

    Plasmid
    #127404
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.
    Depositor
    Sharona Gordon
    Publication
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: E322 to Amber codon and W34…
    Promoter
    CMV
    Available since
    June 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127404-plasmid-map-sequence-id-247056

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  11. DNMT3L-dCas9

    Plasmid
    #126587
    Purpose
    DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3L (DNMT3L Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3L-dCas9-NLS
    Expression
    Mammalian
    Available since
    Dec. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    126587-plasmid-map-sequence-id-244969

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  19. FRB-CFP-ras

    Plasmid
    #87451
    Purpose
    rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane
    Depositor
    Xiaodong Liu
    Publication
    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB (MTOR Human)
    Tags
    CFP and ras fragment
    Expression
    Mammalian
    Mutation
    FKBP and rapamycin binding domain only
    Promoter
    CMV
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87451-plasmid-map-sequence-id-174915

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Showing: 6801 - 6820 of 7076 results