We narrowed to 11,462 results for: ARIA

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LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

Plasmid

#192374

Purpose

To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCStrunc-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K216E]

Plasmid

#188600

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K216E [AAA=> GAA]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625 CM]

Plasmid

#188588

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler C-terminal domain, catalytic mutant

Tags

FLAG-Myc

Expression

Insect

Mutation

D435A [GAT=>GCC]; E435A [GAA=>GCC]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]-Gwpep(Mm)

Plasmid

#188589

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc and GWpep mm

Expression

Insect

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K276A]

Plasmid

#188597

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K276A [AAG=>GCG]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]-Gwpep(Dm)

Plasmid

#188590

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc and Gwpep dm

Expression

Insect

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Gwpep(Dm)-Nbr[C399-625]

Plasmid

#188592

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc-Gwpep dm

Expression

Insect

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Gwpep(Mm)-Nbr[C399-625]

Plasmid

#188591

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc-Gwpep mm

Expression

Insect

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A,K276A]

Plasmid

#188596

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K284A]

Plasmid

#188595

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K284A [AAG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A]

Plasmid

#188594

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K187A]

Plasmid

#188593

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K187A [AAA=> GCA]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr

Plasmid

#188584

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

Plasmid

#158771

Purpose

Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLeGO.sgGata1.4.RUNX1A.iG2

Plasmid

#181976

Purpose

gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

Depositor

Jan-Henning Klusmann

Inserts

GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
RUNX1A (RUNX1 Human)

Use

Lentiviral

Tags

HA

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Sirius-KRasCT

Plasmid

#175831

Purpose

Express Sirius with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-SECFP-KRasCT

Plasmid

#175832

Purpose

Express SECFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-KRasCT

Plasmid

#175836

Purpose

Express iRFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG(Y205F)

Plasmid

#155132

Purpose

Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

Y205F, insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 351

Plasmid

#138058

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-10AG 11AC-GFP

Expression

Bacterial

Mutation

AAA GAG GAG GCA

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 336

Plasmid

#138057

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 2AC 11AC-GFP

Expression

Bacterial

Mutation

GCA GAG GAG ACA

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 333

Plasmid

#138056

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 11AC-GFP

Expression

Bacterial

Mutation

GAA GAG GAG ACA

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 330

Plasmid

#138055

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb- 2AC 11AC-GFP

Expression

Bacterial

Mutation

ACA GAG GAG ACA

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 327

Plasmid

#138054

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 2AC-GFP

Expression

Bacterial

Mutation

GCA GAG GAG AAA

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-Occludin

Plasmid

#136637

Purpose

Expression of Occludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

Occludin (OCLN Chicken)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludin

Plasmid

#136638

Purpose

Expression of HAOccludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludin (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-OccludinCT3

Plasmid

#136639

Purpose

Expression of OccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

OccludinCT3 (OCLN Chicken)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludinCT3

Plasmid

#136640

Purpose

Expression of HAOccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludinCT3 (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbu

Plasmid

#129637

Purpose

Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbu

Tags

His6-tag

Expression

Bacterial

Mutation

V14A and G107H

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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