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pSKAP_AG

Plasmid

#122709

Purpose

pSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AG

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AE

Plasmid

#122707

Purpose

pSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AE

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAW188

Plasmid

#113178

Purpose

E. coli expression vector for untagged E. coli Cas1 R131A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R131A

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHES944

Plasmid

#112642

Purpose

express SST2 from the GAL1 promoter

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

SST2

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAW181

Plasmid

#113127

Purpose

E. coli expression vector for His-MBP-E. coli Cas1 R138A

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Insert

Cas1

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW167

Plasmid

#113187

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 R40E

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R40E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW156

Plasmid

#113185

Purpose

E. coli expression vector for untagged E. coli Cas1 K259E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K259E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW186

Plasmid

#113184

Purpose

E. coli expression vector for untagged E. coli Cas1 R163A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R163A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW151

Plasmid

#113183

Purpose

E. coli expression vector for untagged E. coli Cas1 R146A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R146A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW150

Plasmid

#113182

Purpose

E. coli expression vector for untagged E. coli Cas1 S143A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

S143A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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