We narrowed to 6,905 results for: crispr cas9 plasmids

1. pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

   Plasmid

   #208111

   Purpose

   Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

   Insert

   SpG C, U6, scaffold
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. KTK_095

   Plasmid

   #180524

   Purpose

   dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgRNA-Cre-GpNLuc

   Plasmid

   #208382

   Purpose

   Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

   Plasmid

   #238007

   Purpose

   300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

   Depositor

   Simon Powell, Agnel Sfeir

   Article

   Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

   Insert

   AAVS1 (AAVS1, PPP1R12C Human)
   

   Use

   Rna donor to study rt-dsbr

   Tags

   Expression

   Mammalian

   Mutation

   3bp insertion signature (ATC) at Cas9 cut site in…

   Promoter

   CMV

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

   Plasmid

   #207863

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207860

   Purpose

   AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

   Plasmid

   #207861

   Purpose

   AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Manuscript

   Promoter

   Cbh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP773_pLenti-6xHRBKET-sgRNA

   Plasmid

   #211766

   Purpose

   Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pCMV and U6

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-NQL005-SOX2-sgRNA

   Plasmid

   #175553

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN

    Plasmid

    #220128

    Purpose

    Expresses TadA8e and Sauri cas9N by MYL2 promoter, and add an HA tag fused to TadA

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, TadA, nSauri Cas9N, inteinN
    

    Use

    AAV

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

    Plasmid

    #226915

    Purpose

    Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, TadA, nSp Cas9N, inteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

    Plasmid

    #226916

    Purpose

    Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, inteinC, nSp Cas9C
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    MYL2

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CASI-InteinC-NG C aa714-1368-U6-Lmna sgRNA1

    Plasmid

    #206974

    Purpose

    Expresses NG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    NG aa714-1368, U6, Lmna sgRNA1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1

    Plasmid

    #208110

    Purpose

    Expresses SpG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    SpG C, U6, Lmna sgRNA1, scaffold
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF-LEF and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, Human, S. pyogenes)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid

    #100941

    Purpose

    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    

    Use

    Tags

    1xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pABE8e-NG-403Q-Nterm-AAV

    Plasmid

    #194651

    Purpose

    N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG N-term
    

    Use

    AAV

    Tags

    BPNLS and NpuN spilt intein

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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