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Plasmid#128584PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Glutamine61 to Leucine in the RAC1 moiety.Available SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-P29S-shortlinker
Plasmid#128583PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Proline29 to Serine in the RAC1 moiety.Available SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
loxP-Hygro-loxP-HA-mStayGold-Rab11 HR
Plasmid#229678PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cellsDepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-Halo-ALFA-polyA-G418 HR
Plasmid#229681PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-mStayGold-polyA-Hygro HR
Plasmid#229679PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cellsDepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-SNAP-V5-polyA-Puro HR
Plasmid#229680PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
loxP-G418-loxP-3xALFA-Halo-Rab11 HR
Plasmid#229676PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cellsDepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
loxP-Puro-loxP-3xV5-SNAP-Rab11 HR
Plasmid#229677PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107
Plasmid#64615Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
RKS-Fzd7/8 subtype NGS Wnt
Plasmid#159628PurposeExpress Fzd7/8 subtype NGS Wnt in mammalian cellsDepositorInsertFzd7/8 subtype NGS Wnt
UseLentiviralTagsHis tagExpressionMammalianAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-MPSV-eBFP2
Plasmid#220472PurposeExpresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-SFFV-eBFP2
Plasmid#239601PurposeExpresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selectionDepositorInsertspleen focus‑forming virus - promotor
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
RMCE halo-flag DNA-PKcs
Plasmid#233918PurposeLow copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.DepositorAvailable SinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-hGRAD
Plasmid#207837PurposeInducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsExpressionMammalianMutation215 amino acids of the full length proteinPromoterTRE3G BI promoterAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
RKS-Fzd7 subtype NGS Wnt
Plasmid#159626PurposeExpress Fzd7 subtype NGS Wnt in mammalian cellsDepositorInsertFzd7 subtype NGS Wnt
UseLentiviralTagsHis tagExpressionMammalianAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
RMCE K>R DNA-PKcs
Plasmid#233917PurposeLow copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.DepositorInserthuman DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
ExpressionMammalianMutationK3753RAvailable SinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGBacmam-NGS Wnt-Fc
Plasmid#159629PurposeExpress Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid researchDepositorInsertFzd7/8 subtype NGS Wnt Fc
TagsFc tag and His tagExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-EGFP-ALDH1A3-Neo
Plasmid#189744PurposeThe construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.DepositorAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIGR1-Cdc42-1
Plasmid#179517PurposeRetroviral expression of Cdc42 splice variant 1DepositorAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only