We narrowed to 63,610 results for: cell

1. FUW TetO HA-AMPKa2 AS

   Plasmid

   #69827

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTT3-SP-6xHis-ISLR2(FL)-FLAG

   Plasmid

   #157626

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   ISLR2 (ISLR2 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO5-SgHottip-GFP-CRISPRa

   Plasmid

   #134990

   Purpose

   dCas9-mediated activation of HOTTIP in mammalian cells

   Depositor

   Suming Huang

   Article

   Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

   Insert

   HOTTIP (HOTTIP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGAPTrap-IRESMuro

   Plasmid

   #82336

   Purpose

   Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   IRESMuro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTT3-SP-6xHis-KIR2DL1(FL)-FLAG

   Plasmid

   #157625

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL1 (KIR2DL1 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAPTrap-mtagtdTom_IRESMuro

   Plasmid

   #82355

   Purpose

   Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   mtagTandemTomato-IRESMuro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAPTrap-eGFP-IRESMuro

   Plasmid

   #82504

   Purpose

   Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   eGFP-IRESMuro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

   Plasmid

   #182049

   Purpose

   expression of Sox2 mutants

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S248A/T258A

   Promoter

   CAG

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAPTrap-Luc2-IRESMeo

   Plasmid

   #82509

   Purpose

   Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   Luc2-IRESMeo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGAPTrap-LacZ-IRESMuro

    Plasmid

    #82507

    Purpose

    Vector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    LacZ-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGS-M1-BRG1-3xFLAG-BAF57

    Plasmid

    #109189

    Purpose

    Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 and untagged BAF57 under the control of a p10 promoter in baculovirus/insect cell systems

    Depositor

    Monika Golas

    Article

    Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

    Inserts

    BRG1 (SMARCA4 Human)
    BAF57 (SMARCE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGS-M1-BRG1-3xFLAG-BAF57-BAF60A

    Plasmid

    #109190

    Purpose

    Plasmid for expression of C-terminally 3xFLAG-tagged BRG1, untagged BAF57 and untagged BAF60A under the control of a p10 promoter in baculovirus/insect cell systems

    Depositor

    Monika Golas

    Article

    Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

    Inserts

    BRG1 (SMARCA4 Human)
    BAF57 (SMARCE1 Human)
    BAF60A (SMARCD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-CRT-Fc P

    Plasmid

    #221855

    Purpose

    Express human Calreticulin truncated P domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses P domain

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-CRT-Fc N

    Plasmid

    #221854

    Purpose

    Express human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses N domain

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-CRT-Fc C

    Plasmid

    #221856

    Purpose

    Express human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Expresses C domain

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-3xFLAG-TAF5-∆ex8

    Plasmid

    #209050

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 deltaexon-8
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-miniTurbo-TAF5-∆ex8

    Plasmid

    #209052

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 deltaexon-8
    

    Use

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-3xFLAG-TAF5-FL_WobbleMut

    Plasmid

    #209055

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNA

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 full length
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut

    Plasmid

    #209056

    Purpose

    Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 deltaexon-8
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO inducible promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-858_CD19-BBz_CAR_TFAP4

    Plasmid

    #207503

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-BBz_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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