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psiCHECK-215a-1

Plasmid

#42085

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains the 1259-1281 nt segment of ZEB1 mRNA (N…

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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psiCHECK-640s-2

Plasmid

#42090

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains the 1956-1981 nt segment of ZEB1 mRNA (N…

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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psiCHECK-640s-1

Plasmid

#42089

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains the 1419-1441 nt segment of ZEB1 mRNA (N…

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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psiCHECK-302a-1

Plasmid

#42088

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains the 4055-4078 nt segment of ZEB1 mRNA (N…

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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psiCHECK-640a-2

Plasmid

#42093

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains the 1635-1657 nt segment of ZEB1 mRNA (N…

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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psiCHECK-215a-2

Plasmid

#42086

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains the 2444-2466 nt segment of ZEB1 mRNA (N…

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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GMR-hopscotch

Plasmid

#42062

Depositor

Norbert Perrimon

Article

Bach et al Genetics. 2003 Nov;165(3):1149-66.

Insert

hopscotch (hop Fly)

Expression

Insect

Available Since

Feb. 21, 2013

Availability

Academic Institutions and Nonprofits only
hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Article

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Tags

2X myc

Expression

Insect

Promoter

Hsp70

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
719-2µ-minHIS3

Plasmid

#40610

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pJK520

Plasmid

#155386

Purpose

PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
PphlF-TA1 in dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-WDR24

Plasmid

#87050

Purpose

mammalian expression of WDR24

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

WDR24 (WDR24 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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KG371-GFP-mios

Plasmid

#87052

Purpose

mammalian expression of mios

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

mios (MIOS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pPGK-SB13

Plasmid

#236078

Purpose

SB13 transposase, for use with Sleeping Beauty transponson plasmids

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Insert

SB13 transposase

Use

Sleeping beauty (sb) transposase plasmid

Promoter

PGK

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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MSGV-1D3-28Z All ITAMs intact

Plasmid

#107226

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs intact

Depositor

James Kochenderfer, Steven Rosenberg

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z All ITAMs intact

Use

Retroviral

Tags

None

Mutation

None

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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AY22_pgRNA.R5.1

Plasmid

#100294

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6 RNA Pol III promoter human snRNA

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-p21 promoter-Luc

Plasmid

#33021

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

p21 promoter (CDKN1A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

p21

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

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pUC19-mAirn-R-loop

Plasmid

#134900

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mAirn promoter (Airn Mouse)

Use

Synthetic Biology

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-hSNRPN-R-loop

Plasmid

#134902

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

hSNRPN promoter (SNRPN Human)

Use

Synthetic Biology

Promoter

T7

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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