We narrowed to 6,905 results for: crispr cas9 plasmids

1. Lenti-N1303K-ngRNA+14_EF1a-puroR

   Plasmid

   #207359

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   N1303K-CFTR +14 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-L227R-ngRNA+15_EF1a-puroR

   Plasmid

   #207360

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR +15 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

   Plasmid

   #209787

   Purpose

   Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   TNT4, TadA8e, SpRY N, inteinN, miR122 TS
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TNT4

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

   Plasmid

   #207355

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

   Plasmid

   #163643

   Purpose

   Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON17 hgRNA
   Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
   

   Use

   Lentiviral

   Tags

   Luc2-P2A-tdTomato-T2A

   Expression

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   EFS and pU6

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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