159,481 results

1. ptdTomato-SMCR8

   Plasmid

   #83438

   Purpose

   Expresses SMCR8-tdTomato in mammalian cells

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   Human SMCR8 (SMCR8 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_HEK3+1T>A_(PP7-C4-Q1)

   Plasmid

   #232437

   Purpose

   pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

   Insert

   3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EF1a-Ngn3ER-PuroR-mCherry

   Plasmid

   #197385

   Purpose

   To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   Ngn3ER-2A-PuroR-2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-p53-shRNA-427

   Plasmid

   #25636

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   p53-shRNA-427 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3-EF1aH NICD1

   Plasmid

   #86500

   Purpose

   Mammalian expression of Myc tagged NICD1

   Depositor

   Xin Chen

   Article

   Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

   Insert

   Notch1 (C-term) (Notch1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain only (AA 1753-2531)

   Promoter

   EF1a

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB53x EF1-Dendra2-RPB1Amr

   Plasmid

   #81228

   Purpose

   expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

   Depositor

   Timothée Lionnet

   Article

   Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

   Insert

   Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   N792D alpha amanitin resistance mutation (Bartolo…

   Promoter

   EF1a

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-HMGB2

   Plasmid

   #222532

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   HMGB2 (HMGB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

   Plasmid

   #84561

   Purpose

   pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
   

   Use

   Lentiviral

   Tags

   Flag, Suntag, oxBFP, AID (auxin-inducible degron)

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-inducible Luciferase reporter

    Plasmid

    #64127

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetOx13-CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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