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pcDNA3.1-20nm-EBFP2

Plasmid

#231185

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFB2D

Plasmid

#195286

Purpose

Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFB2E

Plasmid

#195288

Purpose

Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ER-EBFP2

Plasmid

#231183

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-dnMCAK

Plasmid

#205993

Purpose

Over-expression of GFP-dnMCAK

Depositor

Floris Foijer

Article

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

dnMCAK

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-mCherryTRF1-IRES-Blast

Plasmid

#182588

Purpose

expresses mcherry-trf1 in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTRF1 (TERF1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mCherry (JDW 1240)

Plasmid

#224499

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mCherry

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT7_H187Y

Plasmid

#53151

Purpose

expression vector for catalytically inactive human Sirtuin7

Depositor

Katrin Chua

Article

Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

Insert

Sirt7 (SIRT7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H187Y, catalytic inactive

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDiCre-mCherry

Plasmid

#164572

Purpose

Integration of a DiCre cassette in PbGFP parasite genome

Depositor

Olivier Silvie

Article

Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

Inserts

FKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-iCre (JDW 921)

Plasmid

#224519

Purpose

A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP-P2A-iCre

Use

Cre/Lox

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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