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pET21a - Mtb-TF

Plasmid

#111513

Purpose

Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

Trigger Factor

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shB3GNT5.1

Plasmid

#110325

Purpose

TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ140

Plasmid

#99895

Purpose

Empty vector with attL5-attL2 recombination sites for Gateway Cloning

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR and TALEN

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-AC3CtermGenomic-mCherry

Plasmid

#105250

Purpose

mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.1.mKO2

Plasmid

#85209

Purpose

TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYBB3

Plasmid

#80750

Purpose

Expression of human beta B3-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBB3 (CRYBB3 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYBA1

Plasmid

#80745

Purpose

Expression of human beta A1-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBA1 (CRYBA1 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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Gal4pBS12-Pleum

Plasmid

#60326

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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UASGAL-Pleum

Plasmid

#60333

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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FRIG-myc-Sema4D-CD4

Plasmid

#51606

Purpose

expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Human, Mouse)

Use

Lentiviral

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

RSV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

Plasmid

#46839

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-1-290-mcherrry

Plasmid

#249732

Purpose

Expressing human ULK1-1-290 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-290

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ULK1-290-835-mcherry

Plasmid

#249733

Purpose

Expressing human ULK1-290-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

290-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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LgBiT-dSaCas9

Plasmid

#236758

Purpose

Split NanoLuc luciferase biosensor fused to dSaCas9

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc LgBit

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSpCas9-SmBiT

Plasmid

#236759

Purpose

Split NanoLuc luciferase biosensor fused to dSpCas9

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc SmBit

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSaCas9-NLuc

Plasmid

#238947

Purpose

C-terminal fusion of NanoLuc luciferase to dSaCas9, mammalian expression

Depositor

David Segal

Article

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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EML8552_pEML41003-tufApst4-15

Plasmid

#237865

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufA

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufA

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8553_ pEML41003-tufBpst4-15

Plasmid

#237866

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufB

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufB

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ECFP

Plasmid

#247760

Purpose

mammalian expression of ECFP

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

ECFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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