We narrowed to 8,324 results for: gnal

1. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET22b Ag43 160N 6H mCherry-R5 AmpR

   Plasmid

   #225141

   Purpose

   Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

   Depositor

   Urartu Seker

   Article

   Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

   Insert

   Ag43 160N, mCherry
   

   Use

   Tags

   Ag43 protein fused to silaffin-R5 and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SARS-CoV spike all5KO mutant

   Plasmid

   #231921

   Purpose

   For expression of SARS-CoV spike protein with glycan deletion at N1080, N1116, N1140, N1155, N1176 by implementing Asn-to-Gln mutation

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

   Insert

   spike all5KO (S SARS-CoV)
   

   Use

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   N1080Q, N1116Q, N1140Q, N1155Q, N1176Q

   Promoter

   CMV

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEFL-HAx2-TEADiv2

   Plasmid

   #228873

   Purpose

   Expression of optimized TEAD inhibitor (TEADiv2): 2xHA-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

   Insert

   TEADiv2
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

   Plasmid

   #99696

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-NIR-FbLAG30

   Plasmid

   #220748

   Purpose

   NIR-FbLAG30 expression in bacteria.

   Depositor

   Vladislav Verkhusha

   Article

   Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

   Insert

   NIR-FbLAG30
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Bacterial

   Mutation

   For bacterial cytoplasmic expression, the pelB si…

   Promoter

   T7

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-MF3958 Heavy chain-SpyTag003

   Plasmid

   #216303

   Purpose

   Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MF3958 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Tras heavy chain-SpyTag003

   Plasmid

   #216293

   Purpose

   Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-39S Heavy chain-SpyTag003

    Plasmid

    #216296

    Purpose

    Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    39S Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA012

    Plasmid

    #215974

    Purpose

    Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211844

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    UBIQUITIN10

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYSD101

    Plasmid

    #212925

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYSD140

    Plasmid

    #212927

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYSD001

    Plasmid

    #212898

    Purpose

    Encodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA2 (AGA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYSD008

    Plasmid

    #212905

    Purpose

    Encodes S. cerevisiae PLB1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    PLB1 (PLB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYSD006

    Plasmid

    #212903

    Purpose

    Encodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    MFalpha1 (MF(ALPHA)1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYSD003

    Plasmid

    #212900

    Purpose

    Encodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    ECM14 (ECM14 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYSD002

    Plasmid

    #212899

    Purpose

    Encodes S.cerevisiae CRH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    CRH1 (CRH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYSD007

    Plasmid

    #212904

    Purpose

    Encodes S. cerevisiae OST1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    OST1 (OST1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge