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pMXs-LNI (with mNgn2x3HA)

Plasmid

#233195

Purpose

Retroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-LNI

Plasmid

#233205

Purpose

Lentiviral dox-inducible expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

Plasmid

#74306

Purpose

Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Inserts

TRPV4 (Trpv4 Rat)
ferritin (FTL and HTL1) (FTL, FTH1 Human)

Use

AAV and Cre/Lox

Tags

FLAG and p2A

Expression

Mammalian

Promoter

CMV (after cre recombination)

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD2-m1

Plasmid

#114120

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX_307-SOD1E133insTT

Plasmid

#83446

Purpose

Mammalian expression of SOD1E133insTT.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133insTT (after GA)

Promoter

EF-1α

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133Δ

Plasmid

#83445

Purpose

Mammalian expression of SOD1E133Δ.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133Δ

Promoter

EF-1α

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-LNI-bGH

Plasmid

#233203

Purpose

AAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Lhx3, Isl1 Mouse)

Use

AAV

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-NIL (with mNgn2x3HA)

Plasmid

#233192

Purpose

Retroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mNgn2x3HA-P2A-Isl1-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-NLI (with mNgn2x3HA)

Plasmid

#233193

Purpose

Retroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mNgn2x3HA-P2A-mLhx3-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-LIN (with mNgn2x3HA)

Plasmid

#233194

Purpose

Retroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-Isl1-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-ILN (with mNgn2x3HA)

Plasmid

#233196

Purpose

Retroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

Isl1-P2A-mLhx3-T2A-mNgn2x3HA (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-INL (with mNgn2x3HA)

Plasmid

#233197

Purpose

Retroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

Isl1-P2A-mNgn2x3HA-T2A-mLhx3 (Neurog2, Isl1, Lhx3 Mouse)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTL

Plasmid

#203176

Purpose

Centromeric yeast expression vector, leucine selection

Depositor

William Sessa

Article

Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SOD1E133K

Plasmid

#83447

Purpose

Mammalian expression of SOD1E133K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E133K

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

Plasmid

#164458

Purpose

AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minCMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12251-pAAV-AiE0453m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2251)

Plasmid

#164453

Purpose

AiE0453m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

Plasmid

#164457

Purpose

AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minCMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP12254-pAAV-AiE0459m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2254)

Plasmid

#164454

Purpose

AiE0459m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-eGFP-FHA-SHLD2-m1

Plasmid

#114122

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AiP12256-pAAV-AiE0462m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2256)

Plasmid

#164456

Purpose

AiE0462m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge