We narrowed to 8,596 results for: Set

1. pMS17

   Plasmid

   #127088

   Purpose

   Integrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.

   Depositor

   Maggie Smith

   Article

   Rodriguez-Garcia et al Nucleic Acids Res. 2005 May 25;33(9):e87

   Inserts

   tcp830
   TetRiS
   terminator mmrt
   Terminator fdt
   

   Use

   Streptomyce integration vector derived from phic3…

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pre_IV_CS8_7569_v1

   Plasmid

   #126865

   Purpose

   7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS8_7569_v1
   

   Use

   Synthetic Biology

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pALOD4 non-binding mutant

   Plasmid

   #111027

   Purpose

   Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A, G501A, T502A, T503A, L5…

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

   Plasmid

   #91918

   Purpose

   Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LbfCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN1-Lck-GCaMP3

   Plasmid

   #26974

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

   Insert

   Lck-GCaMP3
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Plasmid contains N-terminal 26 amino acid membran…

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4B-Aalb_743

   Plasmid

   #176676

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. T/brachyury(2A-Dre)

   Plasmid

   #140761

   Purpose

   Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   Dre
   

   Use

   Mouse Targeting

   Promoter

   Endogenous T/brachyury

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2340_Tier3(SB)-iRFP_Puro

   Plasmid

   #169638

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ULK1-Halo HRD

    Plasmid

    #207548

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

    Plasmid

    #83947

    Purpose

    gRNA cassette-carrying vector with natMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    gRNA targeting ADE2 gene
    

    Expression

    Yeast

    Available Since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT9624-BEAR-cloning

    Plasmid

    #162986

    Purpose

    BEAR target cloning plasmid with split EGFP

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Halo-TCAB1 HRD

    Plasmid

    #207535

    Purpose

    Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEND-418_Cacnes_LysA

    Plasmid

    #225607

    Purpose

    Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH231-EF1-Puro

    Plasmid

    #115143

    Purpose

    Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Puromycin
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMX-DivisionRecorder

    Plasmid

    #179446

    Purpose

    Retroviral expression vector containing the DivisionRecorder cassette

    Depositor

    Ton Schumacher

    Article

    Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

    Insert

    GFP-P2A-511-CrePI-511
    

    Use

    Retroviral

    Promoter

    LTR

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pATmin-GG

    Plasmid

    #191352

    Purpose

    Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1-Lck-GCaMP2

    Plasmid

    #24794

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

    Insert

    Lck-GCaMP2
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDF0336 pAAV EFS-HvsCas7-11 for dual vector

    Plasmid

    #172515

    Purpose

    Encodes HvsCas7-11 mammalian expression casette in AAV backbone

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV EFS-HvsCas7-11 for dual vector
    

    Use

    AAV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYFAC-CH2

    Plasmid

    #168978

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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